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Client: Cotter Corporation CO-0001244 SCG Project No.: 3101187 <br />Site: Schwartmalder Mine WWTF <br />Fathead minnow Test Results <br />Fathead minnow results are summarized in Table 3 and are provided on data sheets in <br />Appendix 3. Survival was 82% in the 100% effluent concentration and ranged from 80% - 85% <br />in the remaining effluent concentrations. Control survival was 92%. No statistically significant <br />differences for survival were measured in the test. The IC25 for survival was >100%. <br />Table 3. Summary of Fathead minnow test results. Forty fish were exposed at each <br />concentration. An asterisk (*) denotes a statistically significant difference from the control. <br /> No. Avg. Wt. <br />.... Inc <>.."> <br />t eapt* <br /> <br />Concentration <br />Alive <br />tin-g) <br />Min. <br />Max. :.. <br />Control (0%) 37 0.385 0.326 0.437 <br /> <br />12.5% 33 0.368 0.339 0.396 <br /> <br />25% 34 0.365 0.321 0.410 <br /> <br />50% 34 0.342 0.295 0.377 <br /> <br />75% 32 0.347 0.277 0.396 <br /> <br />100% 33 0,339 0.315 0.360 <br />Average weight in the 100% effluent concentration was 0.339mg and ranged from <br />0.342mg - 0.368mg per individual in the remaining effluent concentrations. Average weight for <br />the control fish was 0.385mg for statistical analyses and 0.417mg for test acceptability criteria. <br />No statistically significant differences were measured for growth in any effluent concentration <br />when compared to the control. The IC25 for growth was >100%. <br />Test Acceptability <br />Acceptable control survival was achieved in both tests. Similarly, Ceriodaphnia dubia <br />reproduction and Fathead minnow growth in control organisms met required levels. PMSD was <br />within the required limits for an acceptable test (Table 4). <br />Table 4. PMSD for chronic test parameters. <br />SeaCrest Group 8