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Client: Cotter Corporation CO-0001244 SCG Project No.: 3101187 <br />Site: Schwartmalder Mine WWTF <br />Table 1. Statistics methods used in testing for significant differences in test parameters. <br />Species Normality Homogeneity <br />Ceriodaphnia dubia Kolmogorov Test Bartlett's Test <br />Fathead minnow Shapiro - Wilk's Test Bartlett's Test <br /> Statistical Difference <br /> Survival Growth Reproduction IC25 <br />Ceriodaphnia dubia N/A N/A Dunnett's Test ICp <br />Fathead minnow Dunnett's Test Dunnett's Test N/A ICp <br />RESULTS <br />Ceriodaphnia dubia Test Results <br />Test results for the Ceriodaphnia dubia are summarized in Table 2 and provided on the <br />data sheets located in Appendix 2. Survival was 100% in all effluent concentrations. Control <br />survival was 100%. No statistically significant mortality was measured in any effluent <br />concentration. The IC25 for survival was >100%. <br />Table 2. Summary of Ceriodaphnia dubia test results. Ten animals were exposed at each <br />concentration. An asterisk (*) denotes a statistically significant difference from the control. <br /> u tSi ux ct .: lffere?tc <br /> <br />Concentration No. <br />Surviving Mean <br />Births <br />Min. <br />Max. <br />Sinai"` <br />jE2c zoti.r <br />Control (0%) 10 21.3 12 31 <br />12.5% 10 19.2 14 24 <br />25% 10 19.3 8 26 <br />50% 10 23.2 15 35 <br />75% 10 22.1 14 33 <br />100% 10 20.1 15 30 <br />Average numbers of neonates was 20.1 in the 100% effluent concentration and ranged <br />from 19.2 - 23.2 in the remaining effluent concentrations. Average number of neonates in the <br />control was 21.3. No statistically significant differences in the number of neonates were found <br />between the control and any effluent concentration. The IC25 for reproduction was > 100%. <br />SeaCrest Group 7