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Client: Cotter Corporation CO-0001244 SCG Project No.: 3101187 <br />Site: Schwartzwalder Mine WWTF <br />DISCUSSION <br />A failed test for this discharge occurs whenever there is a statistically significant <br />difference in lethality between the control and any effluent concentration < the IWC of 100% <br />and, the IC25, which represents an estimate of the effluent concentration at which 25% of the test <br />organisms demonstrate inhibition as reflected by lethality, is at any effluent concentration < the <br />IWC. Since neither species had significant mortality meeting these criteria, the discharge passes <br />WET testing requirements. <br />REFERENCES <br />1. Hach Chemical Company. 2008. Hach's Water Analysis Handbook. Fifth Edition. Hach <br />Chemical Company, Loveland, Colorado. Digital Medium. <br />2. APHA/AWWA/WEF. 1998. Standard Methods for the Examination of Water and <br />Wastewater. 20`h Edition. American Public Health Association, Washington, D.C. <br />3. USEPA. 2002. Short Term Methods for Estimating the Chronic Toxicity of Effluents and <br />Receiving Waters to Freshwater Organisms. EPA-821-R-02-013. 335 pp. <br />4. CDPHE (Colorado Department of Public Health and Environment). 1998. Laboratory <br />Guidelines for Conducting Whole Effluent Toxicity Tests. Water Quality Control Division. <br />5. USEPA. 2000. Method of Guidance and Recommendations for Whole Effluent Toxicity <br />(WET) Testing (40 CFR Part 136). EPA/821/13-00/004. <br />6. USEPA. 2000. Understanding and Accounting for Method Variability in Whole Effluent <br />Toxicity Applications under the .National Pollutant Discharge Elimination System Program. <br />EPA/833/R-00/003. <br />SeaCrest Group 9