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COLORADO - CDPS WET TEST REPORT FORM - CHRONIC <br />Permitee: Twentymile Coal Company - Site 1 SP <br />CDPS Number: CO-034142 <br />Outfall: Site 1 SP 2nd Quarter 2010 <br />Type of Test: Routine-Annual Lot #: 061510-1 abe <br />Test Species: Pimephales promelas <br />Test Results Survival Growth Statistical Method <br />IC25 Point Estimate >100% >100% <br /> <br />Pass/Fail <br />PASS <br />NA Linear Interpolation <br />Concentration with Least Statistical Difference >100% 100% Steel's Many-One Rank <br />Pass/Fail PASS NA Dunnett's Test <br />Pimephales promelas Daily Percent Survival (%) <br /> Control 12.5% 25% 50% 75% 100% <br />Initial 100 100 100 100 100 100 <br />Day 1 100 100 100 100 100 100 <br />Day 2 100 100 100 100 100 100 <br />Day 3 100 100 100 100 100 100 <br />Day 4 100 100 100 100 100 100 <br />Day 5 100 100 100 100 100 97.5 <br />Day 6 100 100 100 100 100 97.5 <br />Day 7 100 100 100 100 100 97.5 <br /> <br />7-day mean dry weight (mg) 0.459 0.475 0.470 0.462 0.4°4 0.457 <br /> <br />pH (minimum/maximum) 777/8.3 7.7/8.4 7.7/8.5 7.6/8.5 7.4/8.6 7.3/8.6 <br />Receiving Water used for dilution? NO Total Residual Chlorine: ND <br />Hardness (mg/L) Rec Water -.Nk Effluent :750';50" . MHFSW .-96.99 <br />Alkalinity (mg/L) Rec Water - NA Effluent Kil 622 MHFSW , 57,8V`'. <br />Ammonia (mg/L) Rec Water NA Effluent ND MHFSW i ND <br />Were all test conditions in conformance with division guidelines? YES <br />If "NO", list deviations from test specification: NA <br />Laboratory: Aqua-Tox, Inc. <br />Analyst(s): <br />