2010-08-01_HYDROLOGY - C1981044 - Laserfiche WebLink

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Twentymile Coal Company - Site 5D Chronic Whole Effluent Toxicity Results 2nd Quarter 2010 ATI Lot # 061510-2abc Summary of Laboratory Conditons & Bioassay Results Parameter Ceriodaphnia dubia Pimephales promelas Test Type 7-day static renewal 7-day static renewal Temperature (deg C) 25 +/ - 1 deg C 25 +/ - 1 deg C Light Quality ambient lab levels ambient lab levels Light Intensity (footcandles) 50-100 ft-c 50-100 ft-c Photoperiod 16 hr light/ 8 hr dark 16 hr light/ 8 hr dark Test Chamber Size 40 ml- 500 mL Test Solution Volume 30 mL 300 mL Renewal of Test Solutions every 24 hours every 24 hours Age of Test Organisms less than 24 hours less than 24 hours # of neonates per test chamber 1 10 # replicate test chambers per conc. 10 4 Feeding regime 0.2 mL algaeMC per day 0.15 mL A. naup4l 2xdaily Dilution Water MHFSW MHFSW Test Duration 7-8 days 7-days Effluent Sampling Events and MHFSW Inorganic Chemistries Parameter Sampling Event 1 Sampling Event 2 Sampling Event 3 MHFSW H Dissolved Oxygen (mg/L) 7.72 5.86 7.76 6.84 7.53 6.56 7.95 6.83 Conductivity (pmhos/cm) 1678 1521 1497 309 Ammonia (mg/L) ND ND ND ND Alkalinity (mg/L) 722.63 810.26 783.10 57.81 Hardness (mg/L) Residual Chlorine (mg/L) 365.73 ND 55.71 ND 56.11 ND 96.99 ND Ceriodaphnia dubia & Pimephaies promeias Chronic Statistical Endpoints Species Parameter IC25 NOEC LOEC Ceriodaphnia dubia Survival >100% 100% >100% Ceriodaphnia dubia Reproduction 98.53% 75% 100% Pimephalespromelas Survival >100% 100% >100% Pimephales promelas Growth >100% 75% 100% Ceriodaphnia dubia Survival and Reproduction Data Concentration Number Exposed Percent Survival Mean Average Number of Neonates Control 10 100% 34.8 12.5% 10 100% 35.1 25% 10 100% 34.5 50% 10 100% 33.8 75% 10 100% 33.9 100% 10 1000% 25.7