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COLORADO - CDPS WET TEST REPORT FORM - CHRONIC <br />Permitee: Twentymile Coal Company - Site 1 SP <br />CDPS Number: CO - 034142 <br />Outfall: Site 1 SP 2nd Quarter 2010 <br />Type of Test: Routine-Annual Lot #: 061510-1abc <br />Test Species: Ceriodaphnia dubia <br />Test Results Survival Reproduction Statistical Method <br />IC25 Point Estimate >100% >100% <br /> <br />Pass/Fail <br />PASS <br />NA Linear Interpolation <br />Concentration with Least Statistical Difference >100% >100% Fisher's Exact Test <br />Pass/Fail PASS NA Steel's Many-one Rank Test <br />Ceriodaphnia dubia Daily Percent Survival (%) <br /> Control 12.5% 25% 50% 75% 100% <br />Initial 100 100 100 100 100 100 <br />Day1 100 100 100 100 100 100 <br />Day 2 100 100 100 100 100 100 <br />Day 3 100 100 100 100 100 100 <br />Day 4 100 100 100 100 100 100 <br />Day 5 100 100 100 100 100 100 <br />Day 6 100 100 100 100 100 100 <br />Day 7 100 100 100 100 100 100 <br /> <br />Mean 3 Brood Total 35 r4 34.7 34.8 34.8 33.3 35.6 <br /> J <br />pH (minimum/maximum) 8.0/8.3 7.9/8.4 7.7/8.5 7.5/8.6 7.4/8.7 7.3/8.7 <br />Receiving Water used for dilution? NO Total Residual Chlorine: ND <br />Hardness (mg/L) Rec Water N Effluent 75Q50 `' MHFSW 96':99 <br />Alkalinity (mg/L) Rec Water NA Effluent :,622;69 MHFSW ; '57.81 <br />Ammonia (mg/L) Rec Water NA r Effluent NR MHFSW ND <br />Were all test conditions in conformance with division guidelines? YES <br />If "NO", list deviations from test specification: NA <br />Laboratory: Aqua-Tox, Inc <br />Analyst(s): <br />Jf,