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<br />Narrative and Discussion <br />ADDRESS ALL CORRESPONDENCE TO: <br />COMMERCIAL TESTING 8 ENGINEERING CO. <br />4666 PARIS STREET, SUITE 8-200 <br />DENVER, CO 80239 <br />TEL: (303) 373-4772 <br />FAX: (303) 373-4791 <br />PERMIT AND SAMPLE INFORMATION <br />NPDES permit #: <br />Type of sample: <br />Date and time of sample(s): <br />Date and time of test start: <br />Dilution water: <br />Receiving temperature(s): <br />CO-0032115 <br />Effluent Grab <br />04/14/03 0900 <br />04/15/03 1215 <br />Reconstituted Laboratory Water <br />8.0°C <br />TEST ORGANISMS <br />Fathead minnow Age: 14 days <br />Ceriodaphnia dubia Age: <24 hrs <br />. PROCEDURE <br />Acute, 48 and 96-hour bioassays as outlined in the most recent protocols specified by the Water <br />Quality Control Division of the Colorado Department of Health (1998). • <br />DISCUSSION <br />According to the Trapper Mining Inc. discharge permit (CO-0032115), an acute WET test is failed <br />whenever the LC6°, which represents an estimate of the effluent concentration which is lethal to <br />50% of the test organisms in the time period prescribed by the test, is found to be less than or <br />equal to 100% effluent. <br />By this definition, the results of this bioassay indicate that effluent from outfall 001 is not acutely <br />toxic to fathead minnow and Ceriodaphnia dubia survival. Therefore, outfall 001 is in compliance <br />with the acute WET portion of its Colorado State Discharge Permit for the second quarter of <br />2003. <br />DATE OF REPORT <br />April 20, 2003 <br />Report prepared by: Sarah Lohner <br />Laboratory Technician Date <br />Report reviewed by: Chris Theel <br />Biomonitoring Supervisor Date <br />• <br />4 Comme¢r l 5estinc b Eraineennc Cc. Minerals Service= -Corporate Office <br />1979 S. Hiohland Ave_ Suite 2708. Comhard. IL 60148 t 16301953-9300 116301953-9306 wwwsgs.com <br />rsx, Memnei at the SGS Group ISOCiete Gpnerele de Surveillencel <br />