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<br />1 <br />1 <br />1 <br /> <br />1 <br /> <br /> <br />1 <br /> <br /> <br /> <br />1 <br /> <br />1 <br />1 <br /> <br />J <br />14 BIOlAG1CAL RaroaT 85(1.23) <br />acrylonitriles(Egekezeand Oehme 1980; Purseret <br />al. 1984; Becker 1985; Ballantyne 1987b). <br />Polyacrylonitrile, for example, is used in fabrics, <br />upholstery covers, paddings, and clothing; about <br />50°v of the mass of the pol}•mer is theoretically <br />available as HCA' under thermal decomposition <br />(Purser et al. 1989; Homan 1987). <br />Background Concentrations <br />The reactivity of HCN, and its ability to con- <br />dense with itself and other compounds, was prob- <br />ablyresponsible for the prebiotic formation of the <br />majority of biochemical compounds required for <br />life (Marrs and Ballantyne 1987). Cyanide is now <br />known to be present in a number of foodstuff' and <br />forage plants, as a metabolite ofcertain drugs, and <br />in various industrial pollutants; it also may be <br />formed by the combustion of cyanide-releasing <br />substances, such as plastics in airplane fires and <br />tobacco in smoking (Robinson et al. 1985). Hydro- <br />gen cyanide production may occur in hepato- <br />pancreas of mussels, Mylilus edulis (Vennesland <br />et a1.19816), in rat liver (Solomonson 1981), and in <br />green and blue-green algae during nitrate metabo- <br />lism (Leduc et al. 1982). Except for certain natu- <br />rally occurring organic cyanide compounds in <br />plants, itis uncommon to find c}•anide in foods con- <br />sumed in the United States (EPA 1980). <br />The cyanide anion is found in a variety of <br />naturally occurring plant compounds as cyano- <br />genic glycosides, glycosides, lathyrogenic com- <br />pounds, indoleacetonitrile, and cyanopvridine al- <br />kaloids. Plants that contain cyanogenic g]yco- <br />sidesare potentially poisonous because bruising or <br />incomplete cookie g can result in glycoside hydroly- <br />sisand release of HCN (Towil] et al. 1978). Cyanide <br />concentrations in cyanogenic plants are usual]}' <br />-highest in ]eaves of young plants: 3evels drop rap- <br />idly after pollination (Bieh] 1984). There are about <br />-20 major cyanogenic glycosides, of which usually <br />only one or two occur in any plant. They are syn- <br />thesized from amino acids and sugars and are <br />found in many economically important plants, <br />such as sorghum, flax, lima bean, cassava, and <br />many of the stone fruits (Table 2; Towil] et al. 1978; <br />Shaw 1986). Cassava contains linamurin and <br />lotaustralin, whereas the main cyanogenic <br />glycoside in cereals is dhurrin; consumption of <br />foods containing toxic cyanogens (primarily cas- <br />sava) has been associated with death or mor- <br />bidity-on an acute basis---0r goiter and tropical <br />ataxic neuropathy on a chronic consumption basis <br />lOkolie and Ugochukwu 1989). Cassava is a peren- <br />nial shrub, native to the neotropics, grown for its <br />tuberous starchy roots, and a traditional dietary <br />staple oC many indigenous populations in <br />Amazonia, especially the Tukanoan Indians in <br />northwestern Amazonia (Dufour 1988). Cassava is <br />one of the few food plants in which the cyanide con- <br />tentmay create toxic problems. All varieties otcas- <br />sava contain cyanogenic glycosides capable of <br />liberating HCN, but amounts vary greatly depend- <br />ing on variety and environmental conditions. Bit- <br />ter cultivars of cassava provide over 70°'0 of the <br />Tukanoan's Cood energy, appearing In the diet as <br />bread, meal, a starch drink, and boiled cassava <br />juice. The greatly elevated total cyanide content in <br />bitter varieties (Table 2) may contain 5.1-13.4'i~. of <br />the total as the toxic free cyanide (Dpfour 1988). <br />The production of HCN by animals is almost <br />exclusively restricted to various arthropods: 7 of <br />about 3,000 species of centipedes; 46 of 2,500 spe- <br />cies of polydesmid millipedes; and 10 of 750,000 <br />species otinsects, including 3 species of beetles, 4 <br />moths, and 3 butterflies (Duffey 1981). Milli- <br />pedes-which are eaten frequently by toads and <br />starlings-secrete cyanide for defensive purposes <br />in repelling predators; in zygaenid moths, cyanide <br />seems to be localized in eggs (Tablle 2; Duffey <br />1981). <br />Cyanide concentrations in fish from streams <br />that were deliberately poisoned with cyanide <br />ranged between 10 and 100 µg total cyanide per <br />kilogram whole body fresh weight fFW; Wiley <br />1984). Total cyanide concentrations in gill tissues <br />of salmonids under widely varying conditions of <br />temperature, nominal water concentrations, and <br />duration of exposure ranged from about 30 µg/kg <br />FR' to >7,000 µglkg (Holden and Marsden 1964). <br />Unpoisoned fish usually contained <1 µg/kg FW in <br />gills, although values up to 50 µg/kg occurred occa- <br />sional]}•. Lowest cyanide concentrations in gills oc- <br />curred at elevated (summer) water temperatures; <br />at lower temperatures, survival was greater and <br />residues were higher (Holden and Marsden 1964). <br />Fish retrieved from cyanide-poisoned environ- <br />ments,dead oralive, can probably be consumed by <br />humans because muscle cyanide residues were <br />considered to be low (i.e., <1.000 mg/kg FW; Leduc <br />1954 ~. <br />Cyanide pollution is likely to occur in many <br />places, ranging from industrialized urban areas to <br />gold mines in the western United States and <br />1 <br />