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• _ ~~~ COMMERCIAL TESTING 8~ ENGINEERING CO. <br />~ METHODS <br />A Preliminary Toxicity Identification evaluation was conducted with effluent from Bear Coal <br />Company. An effluent grab sample was collected on July 15, 2002, stored on ice, and delivered <br />to Commercial Testing and Engineering, Co. (CT&E) on the following day. The eflluent sample <br />was received at a temperature of 9.0 ° C and assigned CT&E sample tracking number 072-2777- <br />001. The Initial Toxicity test was initiated approximately 24 hours after sample collection. The <br />characterization tests were initiated approximately 48 hours after sample collection and 24 hours <br />after sample manipulation. <br />Testing was conducted in accordance with the most recent protocols specified by the Water <br />Quality Control Division of the Colorado Department of Health (1998) and with reference to the <br />EPA's Methods for Aquatic Toxicity Identification F_valuations. Phase I Toxicity <br />Characterization Procedures. (EPA/600/3-88/034 September 1988). <br />Basic characteristics of the effluent and dilutions were measured as follows: <br />Characteristic Method/Equipment EPA Method <br />Alkalinity <br />Chlorine, Total Residual <br />Conductivity <br />Dissolved Oxygen <br />Hardness <br />pH <br />Temperature <br />Titrimetric (APHA', 1989) <br />LaMotte STC-Cl Colorimeter <br />Orion Model 160 Cond. Meter <br />Orion Model 850 DO Meter <br />Titrimetric (APHA, 1989) <br />Fisher Mode1925 pH Meter <br />1VIST2 calibrated thermometer <br />EPA 310.1 & 130.2 <br />EPA accepted <br />EPA 120.1 <br />EPA 360.1 <br />EPA 310.1 & ]30.2 <br />EPA 150.1 <br />EPA accepted <br />The Trimmed Spearman-Karber, Graphical Method, Probit, and Binomial statistical program <br />methods determined the LC;o's3. <br />' American Public Health Association <br />' National Institute of Standards and Technology <br />' Lethal Concentrationso - an estimate of [he concentration lethal to 50"/" of the organisms in the time prescribed by <br />the test <br />r-s2s <br />