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METHODS cont. <br />The following tests were conducted in the Preliminary Toxicity Identification evaluation <br />Dav 1 <br />Initial Toxicity Test - C dubia were exposed to 0%, 1.7%, 25%, 50%, 75%, and 100% effluent <br />concentrations prepared using reconstituted laboratory water at 110 mglL CaCOs. Twenty <br />organisms were exposed at each increasing concentration level. <br />Dav 2 <br />Baseline Test (Ceriodaphnia dubia) - C. dubia were exposed to a dilution water blank and <br />50%, 75%, and 100% effluent concentrations prepared using reconstituted laboratory water at <br />110 mg/L CaC03_ Twenty organisms per concentration were exposed approximately 24 hours <br />after the start of the Initial Toxicity Test. <br />pH Adjustment Test (pH 3) -Ten C. dubia were exposed to a dilution water blank and 50%, <br />75%, and 100% effluent adjusted to pH 3 with Hydrochloric Acid (HCl). Prior to organism <br />exposure the effluent was readjusted back to plli' with Sodium Hydroxide (NaOH). Dilutions <br />were prepared with reconstituted laboratory water at 110 mg/L CaC03. <br />Filtration/pH Adjustment Test (pH 3) -Ten C. dubia were exposed to a dilution water blank <br />and 50%, 75%, and 100% effluent adjusted to pH 3 with Hydrochloric Acid (HCl) and filtered <br />through a 1.0 micron glass fiber filter. Prior to organism exposure the effluent was readjusted <br />back to pHi with Sodium Hydroxide (NaOH). Dilutions were prepared with reconstituted <br />laboratory water at 110 mg/L CaC03. <br />Aeration/pH Adjustment Test (pH 3) -Ten C. dubia were exposed to a dilution water blank <br />and 50%, 75%, and 100% effluent adjusted to pH 3 with Hydrochloric Acid (HCl) and aerated <br />for one hour. Prior to organism exposure the effluent was readjusted back to pHi with Sodium <br />Hydroxide (NaOH). Dilutions were prepared with reconstituted laboratory water at 110 mg/L <br />CaC03. <br />Filtration/pH Unadjustment (pHi) -Ten C. dubia were exposed to a dilution water blank and <br />50%, 75%, and 100% effluent without pH adjustment and filtered through a 1.0 micron glass <br />fiber filter. Dilutions were prepared with reconstituted laboratory water at 110 mg/L CaC03. <br />' Initial pH <br />