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METHODS <br />A Preliminary Toxicity Identification evaluation was conducted with effluent from Beaz Coal <br />Company. An effluent grab sample was collected on January 18, 2000, stored on ice, and <br />delivered to Commercial Testing and Engineering, Co. (CT&E) on the following day. The <br />effluent sample was received at a temperature of 7.0 ° C and assigned CT&E sample tracking <br />numbers 72-421348, 72-421349, and 72-421350. The Initial Toxicity test was initiated <br />approximately 24 hours after sample collection. The chazacterizationtesu were initiated <br />approximately 48 hours after sample collection and 24 hours after sample manipulation. <br />Testing was conducted in accordance with the most recent protocols specified by the Water <br />Quality Control Division of the Colorado Department of Health (1998) and with reference to the <br />EPA's Methods for Aquatic Toxicity Identification Evaluations. Phase I Toxicity <br />Characterizafion Procedures. (EPA/600/3-88/034 September 1988). <br />Basic chazacteristics of the effluent and dilutions were measured as follows: <br />Characteristic Method/Equipment EPA Method <br />Alkalinity <br />Ammonia <br />Chlorine, Total Residual <br />Conductivity <br />Dissolved Oxygen <br />Hardness <br />pH <br />Temperature <br />Titrimetric (APHAt, 1989) <br />Lachat Analysis Method <br />LaMotte STC-Cl Colorimeter <br />Orion Model 160 Cond. Meter <br />Orion Model 850 DO Meter <br />Titrimetric (APHA, 1989) <br />Fisher Mode1925 pH Meter <br />NISTZ calibrated thermometer <br />EPA 310.1 & 130.2 <br />EPA 350.1 <br />EPA accepted <br />EPA 120.1 <br />EPA 360.1 <br />EPA 310.1 & 130.2 <br />EPA 150.1 <br />EPA accepted <br />The Trimmed Speazman-Kazber, Probit, and Binomial statistical program methods determined <br />the LCSO's. <br />' American Public Health Association <br />s National Institute of Standards and Technology <br />