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METHODS <br />Effluent and B-seam spring water grab samples from Somerset Mine <br />was collected on October 11, 1993, and received at Commercial <br />Testing and Engineering (CT&E) approximately 24 hours later where <br />they were assigned CT&E number 72-262906. <br />The chemical characteristics of effluent, spring water and <br />laboratory waters were measured. The pH, dissolved oxygen, <br />alkalinity, hardness, conductivity, ammonia and residual chlorine <br />were determined as follows. The pH was measured using a Fisher <br />Scientific model 910 pH meter (EPA method 150.1) and dissolved <br />oxygen was measured with a YSI model 54A oxygen meter (EPA method <br />360.1). Alkalinity and hardness were determined by titrimetric <br />methods specified by the American Public Health Association, 1989 <br />(EPA methods 310.1 and 130.2). Conductivity was measured with a <br />Jenway PCM 3 conductivity meter (EPA method 120.1). Ammonia was <br />measured using 901 Orion ionalyzer and a 901 Orion selective ion <br />electrode (EPA method 350.3). Total residual chlorine was measured <br />using a LaMotte model STC-C1 colorimeter (EPA accepted). Metals <br />scans on effluent and spring water were performed by ARL/Fisons <br />3410 ICP (inductively coupled plasma) and by a Hitachi 28100 (EPA <br />600). <br />Acute tests were conducted in accordance with the most recent <br />protocols specified by the Water Quality Control Division of the <br />Colorado Department of Health, (1), as outlined in Table 1 and, <br />with additional guidance from the Environmental Protection Agency's <br />Methods for Aquatic Toxicity Identification Evaluations, Phase 1 <br />Toxicity Characterization Procedures (2). <br />The concentrations that caused mortality of 50% of the organisms <br />(LCS~'s), were determined with Spearman-Karber statistical analyses. <br />-2- <br />COM MERCIALTESTING & ENGINEE RING CO. <br />~oE ~f~ <br />oinal Wa~erm ark etl Poi Vour Proie coon <br />