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DocuSign Envelope ID: D07924E6-3D94-4398-AO66-B683COBAB33E <br /> 1.0 • O 70 <br /> 0.9 O <br /> 60 <br /> 0.8 <br /> a 0.7 50 <br /> o <br /> 0.6 3 <br /> 9 40 <br /> 2 0.5 0 <br /> 04 rc 30 <br /> 9 9 <br /> 0.3 20 <br /> 0.2 <br /> 0.1 10 <br /> — -- — ---- --------------- <br /> Reject Null <br /> 0.0 0 <br /> 0 D 20 40 60 80 100 0 D 20 40 60 80 100 <br /> Conc-% Conc-% <br /> Figure 5: C. dubia survival, 0.25 g Figure 6: C. dubia reproduction, 0.25g ethanol/g <br /> ethanol/g SO4 SO4 <br /> Table 4: Concentration-response curve evaluation summary. <br /> Treatment Valid Data <br /> Species Endpoint (g ethanol/ Concentration-Response Pattern Curve Acceptability <br /> g SO4) <br /> Survival Varied with significant effects in Yes Approved <br /> the highest test concentration <br /> 0.5 Ideal concentration-response Approved <br /> Reproduction with significant effects in the four Yes with <br /> highest test concentrations Smoothed <br /> IC25 <br /> Survival Varied with no significant effects Yes Approved <br /> C. dubia 1.0 Ideal concentration-response <br /> Reproduction with significant effects in the Yes Approved <br /> three highest test concentrations <br /> Ideal concentration-response <br /> Survival with significant effects in the Yes Approved <br /> three highest test concentrations <br /> 0.25 Varied with significant effects in Approved <br /> Reproduction the three highest test Yes with <br /> concentrations Smoothed <br /> IC25 <br /> GEI Consultants,Inc. 8 February 2021 <br /> Ecological Division Whole Effluent Toxicity Test Report <br /> Stantec-Elk Ridge Mining and Reclamation,LLC <br />