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DocuSign Envelope ID: D07924E6-3D94-4398-AO66-B683COBAB33E <br /> 3.4 Reference Toxicant Summary <br /> Most recent reference toxicant tests met performance requirements (Tables 5-8)................Yes <br /> Most recent reference toxicant tests within control chart requirements...........................Yes/No* <br /> *Survival slightly below performance range for the November C. dubia test. <br /> Table 5: Chronic Ceriodaphnia dubia reference toxicant test with NaCl, conducted November <br /> 3 to 9, 2020. <br /> mg NaCl/L Control 150 300 600 1200 2400 <br /> #alive/#exposed 10/10 10/10 9/10 10/10 2/10 0/10 <br /> % survival 100 100 90 100 20 0 <br /> Normalized 38.5 35.2 33.8 38.9 7.8 0.0 <br /> reproduction ±SD ±3.6 ±6.4 ±7.3 ±2.0 ±9.0 ±0.0 <br /> Normalized Reproduction: <br /> IC25(ICp Method) = 751.1 mg NaCl/L (95% C.I. 701.8 to 808.7) <br /> Note: This is within our accepted performance range (403.4 to 1306.9) <br /> determined by 20 previous reference tests performed. <br /> Table 6: Chronic Ceriodaphnia dubia reference toxicant test with NaCl, conducted January 5 <br /> to 11, 2021. <br /> mg NaCl/L Control 150 300 600 1200 2400 <br /> #alive/#exposed 9/10 10/10 10/10 10/10 9/10 0/10 <br /> % survival 90 100 100 100 90 0 <br /> Normalized 33.5 32.7 28.2 32.3 23.3 0.0 <br /> reproduction ±SD ±6.3 ±6.4 ±10.4 ±3.6 ±10.0 ±0.0 <br /> Normalized Reproduction: <br /> IC25(ICp Method) = 1042 mg NaCl/L (95% C.I. 666.2 to 1347.0) <br /> Note: This is within our accepted performance range (447.9 to 1291.7) <br /> determined by 20 previous reference tests performed. <br /> GEI Consultants,Inc. 9 February 2021 <br /> Ecological Division Whole Effluent Toxicity Test Report <br /> Stantec-Elk Ridge Mining and Reclamation,LLC <br />