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DocuSign Envelope ID: D07924E6-3D94-4398-AO66-B683COBAB33E <br /> 3.3 Statistical QA/QC <br /> Percent minimum significant difference(PMSD) below upper bound (Table 3)..............Yes/No* <br /> *The 0.25 g ethanol/g SO4 test was above the upper bound. <br /> Table 3: Chronic control calculated and allowable PMSD upper bounds by species. <br /> Test Species Treatment Calculated PMSD EPA PMSD Upper <br /> (g Ethanol/g SO4) Bound <br /> 0.5 20 <br /> C. dubia 1.0 24 47 <br /> 0.25 62 <br /> Evaluation of concentration-response curve conducted for all endpoints <br /> (Figures 1 - 6 &Table 4).......................................... ..........................................Yes <br /> 1.0 • O 50 <br /> 0.9 • <br /> 0.8 O 40 <br /> v 0.7 <br /> a e <br /> 0.6 2 30 <br /> 2 0.5 • e <br /> 6 <br /> e 0.4 20 <br /> b <br /> 0.3 <br /> 0.2 10 <br /> 0.1 <br /> 0.0 0 <br /> 0 D 20 40 60 80 100 0 D 20 40 60 80 100 <br /> Conc-% Cono% <br /> Figure 1: C. dubia survival, 0.5 g Figure 2: C. dubia reproduction, 0.5 g <br /> ethanol/g SO4 ethanol/g SO4 <br /> 1.0 • 50 <br /> 0.9 • <br /> 0.8 O O 40 <br /> v 0.7 <br /> a c <br /> 0.6 • • 30 <br /> a a <br /> a <br /> w 0.4 20 <br /> 0.3 <br /> 0.2 10 <br /> 0.1 <br /> 0.0 <br /> 0 D 20 40 60 80 100 0 <br /> 0 D 20 40 60 80 100 <br /> Com-% <br /> Cono% <br /> Figure 3: C. dubia survival, 1.0 g Figure 4: C. dubia reproduction, 1.0 g <br /> ethanol/g SO4 ethanol/g SO4 <br /> GEI Consultants,Inc. 7 February 2021 <br /> Ecological Division Whole Effluent Toxicity Test Report <br /> Stantec-Elk Ridge Mining and Reclamation,LLC <br />