ALP 18

PCC Meeting — October 05, 2010 Version: October 5, 2010
v. E1.6878.2
vi. El. 6882 (normal fill elevation)
3. Fish species tested
i. Fathead Minnow
ii. White Sucker
iii. Largemouth Bass
iv. Bluegill
4. Proceedure
i. Fish transferred to buckets and let rest for 24 hours or more prior to testing
ii. Survivors placed back into 45 gal containers for 96 hour mortality test
iii. Fish and embryos are inserted upstream of sleeve valve through air bleeder
(water to water transfer)
iv. Fish are held there for 30 min to allow for dispersion
(a). For warm water fish water is bled in behind valve for 45 min to 2.5 hours
to allow slow pressurization as to not kill fish. The air bladders are filled
through the blood stream.
1. Cold water fish utilize their mouth to directly pressurize their air
bladders (much faster)
v. Water released through sleeve valve for 5 min of testing.
Results, all fish are ages 0+ and 1+ years
a. June 2009, White Sucker, El. 6775 (27psi)
i. 1+ immediate mortality = 97.43%
(a). 18 survivors but 10 died within 4 days
ii. 0+ immediate mortality = 96.85%
(a). 44 survivors but 27 died within 4 days
iii. l+ total mortality= 98.86%
iv. 0+ total mortality = 98.78%
b. August 2009, White Sucker, El 6795 (36psi)
i. 1+ immediate mortality = 100%
(a). 0 survivors
ii. 0+ immediate mortality = 99.12%
(a). 6 survivors but 2 died within 4 days
iii. 1+ total mortality= 100%
iv. 0+ total mortality = 99.41 %
c. April 2010, Fathead Minnow and Minnow, El. 6805 (41 psi)
i. Bluegill immediate mortality = 9 1. 1 %
(a). 89 survivors but 62 died within 4 days
ii. FHM immediate mortality = 96.15%
(a). 92 survivors but 65 died within 4 days
iii. Bluegill total mortality = 97.3%
iv. FHM total mortality = 98.65%
d. June 2010, Largemouth Bass and Fathead Minnow, El. 6828 (50psi)
i. LMB immediate mortality = 99.6%
(a). 4 survivors but 2 died within 4 days
ii. FHM immediate mortality = 98.73%
(a). 23 survivors but 19 died within 4 days
iii. LMB total mortality = 99.8%
iv. FHM total mortality = 99.78%
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