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population extirpation (Maitland 1995). Hatchery programs are particularly important when <br />habitat alterations, such as those in the Colorado River, prevent wild populations from <br />reproducing and recruiting naturally (Maitland 1995). The goal in maintaining a refugium <br />broodstock and captive propagation program for RBS is to maintain, to the extent possible, the <br />genetic characteristics of wild fish in the captive populations. This requires a captive breeding <br />program that monitors and identifies all critical life history stages at which changes in genetic <br />composition as a result of culture practices may occur (Doyle et al. 1995). <br />This plan lists the genetic risk factors associated with maintenance of captive broodstocks <br />and captive propagation, and outlines strategies to identify, monitor and minimize the risk. <br />Breeding strategies and fish rearing practices are designed and conducted to reduce the effects of <br />drift, selection; inbreeding, outbreeding, and mutational load (Doyle 2001; Doyle et al. 1995)., <br />Maintenance of Genetic Refugia as a Captive Broodstock <br />Over 130 wild adult fish were captured in the early 1980s, and each fish was PIT tagged <br />and transported to Willow Beach NFH and Dexter NFHTC (Hamman 1985). Progeny from those <br />fish represent the `81 broodstock, and the genetic reserves of those wild individuals. Current <br />recovery efforts preclude the process of broodstock mining of adult fish from remaining Lake <br />Mohave RBS populations. However, the ongoing collection of naturally produced larvae from <br />throughout Lake Mohave provides a means of increasing the survival of naturally recruited fish, <br />and a protocol to ensure the genetic diversity of the RBS in Lake Mohave is adequately sampled. <br />For example, adult RBS congregate on spawning bars throughout the lake; larval fish taken from <br />those areas, if sampled throughout the spawning period, should reflect the genome of the current <br />Lake Mohave RBS reproducing population. <br />Fry taken from spawning sites that are destined for future broodstock are grown in aquaria <br />until large enough to PIT tag. Current studies at Dexter NFHTC are examining the use of VIE <br />20