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(visible implant elastomer) tags on smaller life stages of RBS which will allow early mixing of <br />family lots, while still enabling identification at a later date. If the implants are retained, this <br />technique would allow more efficient use of rearing space and mixing of family lots would allow <br />for the use of culture methodologies that more closely resemble a natural habitat. <br />"Nature's" rearing is a culture methodology developed in the Pacific Northwest on salmon <br />hatcheries. This practice advocates that by mimicking the natural environment hatchery fish have <br />1) reduced hatchery selection for potential fitness related parameters, such as light tank <br />backgrounds when the stocking environment is dark, and 2) enhanced survival of hatchery reared <br />fish by imprinting hatchery fish to an appropriate environment. Dexter NFHTC's large earthen <br />ponds serve as `Nature's rearing habitat for RBS. Before RBS are stocked into ponds, vegetation <br />is allowed to flourish, and the ponds are fertilized to enrich the plankton food base. Aquatic <br />insects inhabit the ponds and serve as both food and predators. Bird predation is also present, as <br />are other predators such as raccoons and turtles. This practice should provide a method of pre- <br />acclimation for cove or backwater environment where the fish are to be stocked. The sooner fry <br />can be moved into these rearing environments, the greater the potential for imprinting young fish <br />to a naturalized environment. VIE tagging would allow the earliest transition possible to the <br />`Nature's environment. <br />Non-lethally sampled tissues will be taken from each potential broodfish at Dexter, and <br />those brought into the facility in the foreseeable future. Tissues will be used for PCR-based DNA <br />technologies in genetic identification, characterization and monitoring (Amos and Hoelzel 1992; <br />Ferguson et al. 1995; O'Reilly and Wright 1995). Genetic data will facilitate the management of <br />broodstock and provide guidance for propagation activities. <br />The goals for maintaining a ref igia and broodfish population of RBS at Dexter NFHTC <br />are based on conservation genetic principles as outlined by current conservation genetics theory <br />(Blouin et al. 1996; Lynch 1996; Ford 2002; Lande 1995; Doyle 2001). Although the statistical <br />21