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6) Establish a breeding program, using a genetic database, to maintain and ensure that the <br />effective population size of broodstock is adequate to offset the results of genetic drift in small <br />populations. <br />7) Establish a genetics monitoring program to assess the long-term genetic integrity of production <br />fish. <br />8) Address biological opinion requirements for captive propagation and augmentation. <br />9) Refine spawning, maintenance, and marking techniques of RBS to ensure genetic longevity of <br />the stocks. <br />10) Identify locations, augmentation needs, and current broodstock production capabilities. <br />Genetic Considerations in RBS Broodstock Management and <br />Captive Propagation Program <br />Maintenance of current levels of genetic variation <br />The overall goal of genetics management of native fishes is to protect natural variation <br />and patterns of genetic diversity, and to prevent extinction as a result of deleterious changes to the <br />genome by anthropogenic actions. This plan calls for specific genetics management objectives to <br />maintain existing levels of genetic diversity of RBS throughout the process of broodstock <br />maintenance and captive propagation. <br />Hatcheries, through captive breeding, have prevented the extinction of many species of <br />imperiled fish. Captive breeding and rearing have enhanced wild populations and prevented <br />19