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• population. A baseline genetic analysis will assist in understanding the status. Other information used for status determinations are life history data, vital population statistics and demographic trends, habitat conditions (e.g., carrying capacity of the system), and threats to the species. An exception is where experimental introductions are required to answer questions concerning habitat suitability, and fish behavior. In such cases, limited introductions to address specific critical issues will be approved by the Regional Director, Region 6 if the risks, both genetic and non-genetic, from the action are considered and reduced to acceptable levels. C. Describe the factors which have limited natural reproduction. I.V. Approach: A. If the goal is to re-establish extirpated populations or augment a seriously depleted population, then suitable habitat must be identified in order for the introduction to have a reasonable chance of success. Habitat suitability, therefore, is a vital consideration in setting the priority for all introductions and evaluating the plan. • B. Conduct a risk assessment of the reintroduction/augmentation program associated with the loss of genetic variation and ecological threats. Genetic risks are classified and evaluated based on the potential for interbreeding and hybridization. Ecological threats are usually associated with (1) trophic alterations, (2) spatial alterations, (3) altered growth and survival characteristics, (4) displacement, replacement, or extinction, (5) disease and parasite transmission resulting in epizootic infestation. Disease-free certification is required for all reintroduction/augmentation programs involving endangered fish species. C. Provide specific information on the number and length of fish to be introduced at each location. D. Provide specific dates the fish are to be introducted at each location. If these same locations are to be used several times a year or over a number of years, identify the frequency. It will be necessary to develop long term introduction schedules with annual review and appropriate revisions. is E. Provide specific information on the source of all fish used as broodstock. This will include: wild/hatchery, source of the broodstock, how long have they been at the hatchery, eggs collected from wild fish, etc. Region 6 Fisheries is preparing Genetice Management Guidelines for use on all recovery implementation programs. These guidelines will be used to direct all future broodstock programs within Region 6.