7145 ‎(2)‎

• UCREFRP Catalog Number: 7145
• Author: Inslee, T. D.
• Title: Spawning of Razorback Suckers
• USFW Year: 1981.
• Copyright Material: NO

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back suckers. One pair of fish served as centr.ols, two pair of fish were
injected with 2 mg carp pituitary per pound, and one pair of fish was in-
jected intraperitoneally with 800 IU chorionic gonadotropin. Each female
was reinjected the second and fourth day with the initial dosage.
Hormone Injections and Stripping
Four females were injected with 500 IU chorionic gonadotropin and four
females with a carp pituitary 2 mg/lb. Each fish was checked daily for ripe-
ness. When eggs were available they were stripped, fertilized and placed in
hatching equipment. These fish had been held in the holding house tanks for
about 17 days before injections were started.
Males were held in a seperate tank and injected with 300 and 500 IU
chorionic gonadotropin intermuscularly
Injections were made whenever the
males appeared to be 'losing fluidity.
Fish were seined in Pond C5 about 30 days after stocking and four gravid
females were moved to a holding house tank. One female was injected inter-
muscularly with 500 IU chorionic gonadotropin before being placed in a six
foot circular tank with 3 fluid males that were injected with 300 IU hormone
intermuscularly.
One female was injected with 300 IU hormone and one female was injected
with 500 IU hormone in the muscle. The last female was injected with 2 mg
carp pituitary per pound of body weight daily. A total of three injections
were given each female. The females were held in a holding house tank seper-
ate from males and were checked daily for ripeness.
Egg Hatching
I
Eggs were incubated utilizing five methods: McDonald jars; in the cir -
cular tank on rocks after natural spawning; in a circular tank after stripping;
on a fine mesh net suspended in holding house tank; and in a Heath incubator.
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