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. •T ~•~,l <br />7 <br />Spawning in a Flow-Through Pond <br />Pond D was prepared by placing two overlapping sheets of pressed fiber- <br />glass cloth, 16 feet wide and 27 feet long, across the shallow end of the pond <br />making a liner about 31 x 27 feet. About six yards of rock ranging from ~" <br />to 4" in diameter was scattered evenly over the liner. The pond was filled <br />to a level that covered the rock area from 8" to 24". A three inch pump was <br />used to wash the rocks as the pond was being drained. The pond was refilled <br />and stocked with five females and five males. The adults were seined nn <br />March 5 or 4$ days after they had been stocked in 5C. The females did not <br />appear to be as gravid as would have been desired and it was questionable if <br />spawning would occur. <br />Circular Tank Spawning <br />• Three ripe females and males were placed in each of two six-foot circu- <br />lar tanks, with a water depth of 22 inches. The females in tank ail were in- <br />jected intraperitoneally with dried carp pituitary at 2 mg/lb body weight. <br />The males were injected with. 800 IU human chorionic gonadotropin per pound <br />of body weight. The females were injected daily for three days whereas the <br />males were injected only once. The fish in tank X12 received no hormone and <br />served as a control. Each tank had about 20 gallons of rock, from to 3" <br />diameter, covering the bottom of the tank. A flow of 10 liters per minute <br />of well water (62±10F) was delivered under pressure and aimed at about a 450 <br />angle to cause a circular flow. <br />Tank (Holding House) Spawning <br />A bed of rocks and gravel 4" deep was placed in the upper one-quarter <br />of each of four (30''. x 24"•' x 10') holding house tanks:' Water was flowed at <br />15 gmp through the tank. The water was delivered through an outlet that was <br />below the level of rock bed. Each tank was stocked with one pair of razor- <br />- 2 <br />