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Bruce McCloskey <br />9 June 1995 <br />Page 2. <br />6 temporary FTE months will be needed by Pete to perform the pathology analysis. <br />(2) A research project that will determine the susceptibility of the three native cutthroat <br />trout subspecies along with brook trout, other wild rainbow trout strains, grayling <br />and whitefish will be conducted. If less susceptible (resistant) species or subspecies <br />are identified, genetic analysis will be performed in an attempt to distinguish these <br />strains for future management consideration. Barry will be responsible for the field <br />investigations and Pete will conduct similar controlled experiments and perform the <br />pathology analysis for both the field and laboratory experiments. An additional <br />$10,000 or 5 months of temporary FTE months will be needed by Pete to provide <br />the disease analysis. <br />(3) A research project that will survey known Colorado River native cutthroat trout <br />populations to determine the distribution and abundance of tubifex worm habitat <br />and density of the worms if such habitat is found. It is assumed if tubifex exists <br />in cutthroat trout habitat the risk of those populations becoming infected is greater <br />than if such habitat is not present or is very limited in abundance and distribution. <br />While sampling these habitats, the trout populations will also be sampled for <br />disease analysis and to see if there are any missing age groups. In addition this <br />project will provide CSU geneticists with samples to test their DNA techniques on <br />the worms. This portion of the project will also help answer the question of <br />whether there are other alternate Oligochete hosts besides tubifex worms. If such <br />techniques can be perfected then habitats could be tested for the presence of WD <br />spores without having to test the fish which requires at least four months exposure <br />to the spores and is the most time and resource consuming. Mary McAfee has <br />been reassigned to conduct the field portion of this project along with 6 temporary <br />FTE months, Anita Martinez will coordinate the genetic analysis and Pete will <br />perform the disease analysis. An additional $6,000 or 4 temporary months will be <br />needed by Pete for this project. <br />(4) Hatchery infectivity study will also be conducted to identify spore levels at each <br />WD+ unit. Pete will be responsible for the disease analysis and the Research <br />hatchery will be responsible for collecting the fish for analysis. An additional <br />$13,000 dollars or 7 temporary months are needed for this project. <br />(5) A research project to investigate the dissolved gas effect in the Colorado River has <br />been initiated through the Colorado Cooperative Research Unit at CSU. This <br />project is costing $33,700 this year. Pete will lead a graduate student project <br />investigating the relationship between gas bubble disease and WD in controlled <br />experiments using a part of Pat Davies Toxicology Laboratory (a redirection of <br />resources). In addition Barry will be monitoring gas saturation at each of the <br />rivers listed under the first project. An additional $36,500 and 9 temporary months <br />are needed to fund the graduate study and do the pathology analysis. <br />65