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Correspondence Only <br />Appendix D <br />STATE OF COLORADO <br />DIVISION OF WILDLIFE <br />DEPARTMENT OF NATURAL RESOURCES <br />DATE: 9 June 1995 <br />TO: Bruce McCloskey <br />FROM: Eddie Kochman <br />SUBJECT: Whirling Disease Research Projects <br />At the May 31st Whirling Disease Committee meeting the following research projects were <br />selected and will be initiated as soon as sufficient resources are acquired.. Significant redirection <br />of resources has been accomplished. Additional resources are also required. One project <br />requires a contract with CSU and and sufficient money is not available in the current fiscal year <br />to complete the work. If awarded, the contractor should be able to provide a quick test using <br />DNA analysis to determine if whirling disease organisms are present in water samples, tubifex <br />worms, and small fish under five months of age after refining a new technique. The contractor <br />will be required to assist in the analysis of samples as dictated by research and management <br />objectives using the new technique. Southwest Region Fisheries has agreed to supply $14,000 <br />this year and I recommend we use $6,000 from the Coldwater Fisheries Donation fund to begin <br />the contract. An additional $10,000 will be needed in 1995-96. <br />Following are the research projects that will be accomplished if sufficient resources can be <br />acquired. The activities believed to be top priority are listed below followed by tables showing <br />the resources that have been redirected in an attempt to accomplish these projects and the <br />additional resources needed. <br />(1) A research project has been designed to answer the question of whether the <br />continued stocking of trout from WD+ units increases or maintains WD spore <br />levels in WD+ flowing waters above levels produced by the alternate tubifex worm <br />host. Barry Nehring has been given resources taken from other research projects <br />to conduct the field portion of this project and Pete Walker will perform the <br />pathology analysis. It is expected that this project will take at least 4 years as WD <br />spore levels will be monitored in trout populations where: (A) wild rainbow trout <br />populations are known to be WD positive, year classes are missing and stocking <br />of WD+ rainbow trout is continued. (B) Wild rainbow trout populations are known <br />to be WD positive and there are no missing year classes and WD positive rainbow <br />trout will continue to be stocked, followed by no stocking. (C) Wild rainbow trout <br />populations known to be WD +, year classes are missing and no continued stocking <br />of WD+ ftsh followed by stocking WD+ fish. Susceptible size rainbow trout <br />will be placed in cages to be sentinels for pathology analysis in selected streams. <br />These fish along with sampling the feral population of trout in each stream will <br />provide the information that will answer the question. An additional $11,500 or <br />64