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Bruce McCloskey <br />9 June 1995 <br />Page 3 <br />(6) Distribution and identification of specific sights positive for WD will be conducted <br />by assaying spore levels in specimens collected by regional biologists. Pete will <br />be responsible for this on-going disease analysis. On-going additional costs <br />experienced for the past year have been $57,500 and 27 months of temporary time <br />and hiring a qualified pathologist through a contract with CSU at a cost of <br />$11,000. <br />Resources redirected to accomplish WD research and hatchery clean-up priorities <br />ACTIVITIES - DOLLARS FTE MONTHS SOURCE <br />DNA Analysis for WD/CSU Contr. $20,000 0 SWR& Donation fund <br />R. Judy UV System 250,000 0 Hatchery Cap. Const. <br />Pathology Lab Equip. 23,000 0 Hatchery Cap. Equip. <br />Species Susceptibility 6,000 4 Aquatic Research <br />CRN Habitat/tubifex (field) 23,600 8 Aquatic Research <br />Gas Saturation (field) 33,700 1994-95 Aquatic Administration <br />GBD/WD (lab) 10,000 2 Aquatic Research <br />Genetic Analysis 14,000 <br />Hatchery Sample Collection 19,000 <br />Temporary Pathologist Salary 11,000 <br />Spore Loading/Gas Satu.(field) 121,000 <br />U. N. Carolina Sample Analysis 13,000 <br />Pathologist salary/CSU 11,000 <br />Total $555,300 <br />6 Research Hatchery <br />6 Research Hatchery <br />0 Pathology lab <br />18 Aquatic Research <br />1994-95 Aquatic Administration <br />0 Research Hatchery <br />44 <br />Additional Resources needed in 1995-96 to accomplish WD research priorities. <br />ACTIVITIES DOLLARS TEMP. FTE MONTHS <br />DNA Analysis/CSU Contr. $10,000 12 <br />On-going Path. Lab duties 57,500 27 <br />Species Susceptibility (lab) 10,000 5 $1,000 histopathology <br />CSU Contract for Pathologist 11,000 0 <br />CRN Habitat/tubifex (lab) 6,000 4 <br />GBD/WD Lab Experiment 36,500 9 $20,000 Grad Study <br />Spore Loading (lab) 11,500 6 $1,500 histopathology <br />Hatchery Infectivity 13,000 7 <br /> Total $155,500 70- 5.8 FTE <br />Total cost of redirected and needed money without counting all the administrative time that will <br />be spent by you, myself, Don Horak, Larry Harris, Tom Powell, and others is $710,800 of <br />which Aquatic Resources has redirected enough resources to cover 78 % and are requesting 22 % <br />be provided through additional allocations. In Summary we need an additional $155,500 and <br />70 temporary months. <br />66