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integrate sensory inputs from olfactory receptors and signal <br />detection of an odorant by performing ecologically relevant <br />behaviors (e.g., predator avoidance or initiating searching <br />behavior for food). <br />Behavioral Assay <br />The majority of behavioral studies involving copper and zinc <br />have emphasized estimation of thresholds for toxicant avoidance <br />or preference using previously unexposed fish. Lowest observed <br />effect concentrations for avoidance range from 0.04 to 6.4 µg/L <br />copper (Sprague 1964; Westlake et al. 1974; Folmar 1976; Hara <br />1981; Giattina et al. 1982), and 5.6 to 53 µg/L zinc (Sprague <br />1964; Sprague 1968; Black and Birge 1980). These studies assume <br />that wild fish inhabit uncontaminated water and have the <br />opportunity to avoid contaminant exposure. This assumption may <br />be false for rivers occupied by Colorado squawfish where <br />background concentrations of copper and zinc equal or exceed <br />reported thresholds for avoidance for other fishes (Table 1). <br />Studies in which fish were pre-exposed to copper or zinc, <br />and then challenged with an odorant that elicits a characteristic <br />response, have been conducted less frequently. Saucier et al. <br />(1991) exposed rainbow trout (Oncorhynchus mykiss) to 22 µg/L <br />copper for 37 to 41 weeks and observed that exposed fish lost <br />their ability to discriminate their own rearing water from well <br />water or water containing largemouth bass (Micropterus <br />salmoides). Rehnberg and Schreck (1986) exposed coho salmon <br />14 <br />