9424

• UCREFRP Catalog Number: 9424
• Author: Morizot, D. C., J. H. Williamson and G. J. Carmichael
• Title: Biochemical Genetics of Colorado Pikeminnow
• USFW Year: 2001
• USFW - Doc Type: North American Journal of fisheries Management
• Copyright Material: YES

!' '72 MORIZOT ET- AL.
_~~
'~ ~r~ ~~
'''
TABLE 6.-Allele frequenci
es of seven polym
orphic protein-coding
loci reso
lved in age-0 Colorado pikeminnow
: ' 1,~^I populations. Abbreviations of collection sites are as follows: UGR =upper Green River, MGR =middle Green River,
•' ~ LGR =lower Green River, CAT =Cataract Canyon of the Colorado Ri ver, LCR =lower Colorado River, and MUCR

.
~T =middle and upper Colorado River. No signific ant differences were found among allele frequencies at the six sites.
~a
~¢~= ~ Significant deviations from Ha rdy-Weinberg equi libria (P < 0.01) are i ndicated in sample size data with an asterisk.
' Locus,
~ sample
~
l~ size,
alleles, Population
~
+, ' and mean
FST
UGR
MGR
LGR CAT
LCR
MUCR FsT
~,-~, =:
~--r _ EST-2*
~' N 99 18 122 13 163 3 0.089
- ~ a 0.722 0.583 0.689 0.654 0.629 1.000
- a
~` b 0.273 0.417 0.299 0.346 0.368
03 0.000
0
000
-
' c 0.005 0.000 0.012 0.000 0.0 .
-` ~' GPI-I
"~~~ " N 101 18 ]22 13 169 3 0.018
_
a-
~;
~~
a
0.361
0.417
0.311 0.308
0.370
0.500
`
- 6 0.639 0.583 0.689 0.692 0.630 0.500
GPI-2*
ti-t,~ ~, N 101 18 122 13 169* 3 0.034
a 0.020 0.056 0.041 0.115 0.044 0.000
6 0.980 0.917 0.959 0.846 0.923 1.000
c 0.000 0.000 0.000 0.000 0.030 0.000
d 0.000 0.028 0.000 0.038 0.030 0.000
~,
GR*
N 38 18 113 3 137 3 0.008
1 a 0.987 1.000 0.991 1.000 1.000 1.000
6 0.013 0.000 0.009 0.000 0.000 0.000
PEPB*
N 100 18 122* 13 168* 3 0.009

I 6 0.985 0.972 0.971 1.000 0.979 1.000
'r c 0.015 0.028 0.029 0.000 0.021 0.000
PGAM-2*
N 101 18 117* 13 168 3 0.025
a 0.010 0.028 0.081 0.038 0.074 0.000
6 0.990 0.972 0.919 0.962 0.926 1.000
TP/-2*
N 101 18 122 13 169 3 0.014
a 0.005 0.000 0.012 0.038 0.021 0.000
b 0.995 1.000 0.984 0.962 0.979 1.000
6 C 0.000 0.000 0.004 0.000 0.000 0.000
Mean FsT 0'~
in this study, but the deviation at GPI-2* in the
Colorado River was found again. The mean FsT
on the combined data (0.003) indicated minimal
genetic differentiation among sampled popula-
tions.
~I Hatchery Populations
Analyses of samples taken from two Dexter fish
' technology center broodfish populations indicated
that genetic variability is present, that some gene
frequencies differed, that there are uncommon al-
leles but no H- W equilibrium deviations, and that
moderate genetic divergence is present. Five of
eight informative loci were polymorphic in at least
one hatchery population (Table 8). The frequencies
of the rarely encountered alleles, GPI-2 *a and
PGAM-2 *a, were significantly higher in the brood-
fish derived from the Yampa River (Yampa DX-
F~ [74]) than in those derived from the Colorado
and Green rivers (Colorado-Green DX-Ft [81])
or in combined wild adults (P < 0.05). No sig-
nificant deviations from H-W expectations were
observed in the two Dexter broodfish populations,
in contrast to samples from wild populations
which, when combined, deviated at GPI-2* (Xz =
26.4, df = 3, P = 0.0001). The high frequencies
of alleles rarely encountered in wild populations
(notably GPI-2*a) resulted in a mean FsT (0.108)
among hatchery and wild populations that suggests
moderate genetic differentiation.
Tas[.E
tem-codir
Colorado
from this
A&M). 1\
lele frequ
River cor
Hardy-W,
indicated
icant devi
lows: P <
Locus,
sample
size,
alleles,
and mean
F'ST
EST-2*
N
*a
*b
*c
bGAL*
N
*100
*253
GPI-I*
N
*a
*b
GPI-2*
N
*a
*h
*c
*d
GR*
N
*a
*1i
LDH-C*
N
*-29
*-100
sMDH-2*
N
*100
*103
PEPB*
N
*b
PGAM-2*
N
*a
TPI-1
N
*100
*I02
TPI-2*
N
*a
*b
*c
Mean FST