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and ~od- DX- rado _81~) sig- were ions, lions Xz - ncies ttions .108) ;gents BIOCHEMICAL GENETICS OF COLORADO PIKEMINNOW TABLE 7.-Allele frequencies of 11 polymorphic pro- tein-coding loci resolved in combined Green River and Colorado River age-0 Colorado pikeminnow populations from this study and that of Ammerman and Morizot (1989; A&M). No significant differences were found among al- lele frequencies between the Colorado River and Green River combined populations. Significant deviations from Hardy-Weinberg equilibria (P < 0.01) in this study aze indicated next to sample size (N) with an asterisk; signif- icant deviations in the A&M study are indicated as fol- lows: P < 0.05*, P < 0.01**. Locus, po ulation 73 TABLE 8.-Allele frequencies of five polymorphic pro- tein-coding loci resolved in Dexter National Fish Hatchery and Technology Center, New Mexico, broodfish popula- tions sampled in 1992 and four combined wild adult pop- ulations (Yampa, Green, Colorado, and San Juan rivers) of Colorado pikeminnow. Broodfish population designa- tions are Yampa DX-Fl (74) and Colorado-Green DX-Fl (81); see Table 1 for details. Significant differences in al- lele frequencies are indicated with an asterisk next to the most common allele. Significant deviations from Hazdy- Weinberg equilibria (P < 0.01) are indicated next to sam- ple size (N) with an asterisk. sample p Locus size, Colorado Green , po ulation sample p alleles, River, River, Colorado Green size, Colorado- and mean this this River, River, Combined alleles, Yampa Green FsT study study A&M A&M FsT and mean DX-Ft DX-Ft Combined EST-2* FsT (74) (81) wild FST N 179 239 26** 26* 0.004 EST-z* *a 0.637 0.695 0.577 0.577 N 29 30 145 0.061 *b 0.360 0.297 0.423 0.365 *Q 0.810 0.950 0.731 *~ 0.003 0.008 0.000 0.058 *b 0.190 0.030 0.262 bGAL* *c 0.000 0.017 0.007 1V 26 26 GPI-! *!00 0.654 0.635 N 29 30 169 0.106 *253 0.346 0.365 *a 0.448 0.100 0.376 GP!-!* *b 0.552 0.900 0.624 N 185 241 26 26 0.001 Gp!_2* *a 0.368 0.340 0.365 0.250 N 29 30 165* 0.209 *b 0.632 0.660 0.635 0.750 *a 0.345 0.000 0.033 GPI-2* *b 0.638* 1.000 0.952 N 185 241 26** 26 0.007 *c 0.000 0.000 0.000 *a 0.049 0.033 0.019 0.000 *d 0.017 0.000 0.015 *b 0.919 0.965 0.942 1.000 pgpg* *c 0.003 0.000 0.000 0.000 N 26 29 156 0.007 *d 0.030 0.002 0.038 0.000 *a 0.000 0.000 0.003 GR* *b 1.000 0.983 0.994 N 143 169 0.004 *c 0.000 0.017 0.003 *a 1.000 0.991 PGAM-2 * *b 0.000 0.009 N 29 30 169 0.065 LDH-C* *a 0.207 0.033 0.059 N 26 26 *b 0.793* 0.967 0.941 *-29 0.160 0.000 Mean FsT 0.108 *-l00 0.840 1.000 sMDH-2* N 26 26 ~ *!~ 0.923 0.885 C *!03 0.077 0.115 DISCUSS10I1 PEPB* Our results and analysis of gene frequencies and N 184* 240* 0•~ F sug est that Colorado ikeminnow o ula- *b 0.981 0.977 sT g p p p *c 0.019 0.023 lions we sampled are functioning as a panmictic PGAM-2* group, that is, we cannot reject the null hypothesis N 184 236* 0.003 of panmixia. This suggestion is supported by the *a 0.929 0.953 *b 0.071 0.047 lack of significant differences in allele frequencies TP/-!* among wild populations and by the low mean FsT N 185 2a1 z6 26 values for all polymorphic loci. Similar allele fre- *!00 1.000 1.000 1.000 0.981 *!oz 0.000 0.000 0.000 0.019 quency differences and low mean FsT values have TP!-z* previously led to conclusions of panmixia (Shaklee N 185 2a1 z6 26 0.002 et al. 1990). However, the presence of five possibly *a 0.022 0.008 0.038 0.000 basin-specific alleles (GPI-2*c GR*b PEPS*a *b 0.978 0.990 0.962 1.000 , , , *c 0.000 0.002 0.000 0.000 PEPS*b, and TPI-2*c), albeit at low frequencies, Mean FsT 0.003 plus two other potentially basin-specific alleles (TPI-1 *102 and LDH-3*29) identified by Ammer- ~~=:; - ~.:'- ~; 1v '~Ih,~," ~r 4. I+ _'~`;. t I Vic, 'In 5~. i ~y