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<br />i,
<br />BIOCHEMICAL GENETICS OF COLORADO PIKEMINNOW 71
<br />to TwBt,e 4.-Allele frequencies of five polymorphic pro- Twat.e 5.-Allele frequencies of six polymorphic pro-
<br />m tein-coding loci resolved in adult Colorado pikeminnow tein-coding loci resolved in juvenile Colorado pikeminnow ~,~,'.
<br />
<br />~ populations collected from four rivers (N = 169). No sig- populations collected from three locations in the Colorado ~ ~;~
<br />- nificant differences were found among Colorado, San River. No significant differences were found among Col- ~ ~
<br /> Juan, Green, and Yampa River allele frequencies. Signifi- orado, Green, and Yampa River allele frequencies
<br />Signif-
<br />_ ~ cant deviations from Hardy-Weinberg equilibria (P < .
<br />icant deviations from Hardy-Weinberg equilibria (P <
<br /> 0.01) are indicated next to sample size (N) with an aster- 0.01) aze indicated next to sample size (N) with an aster-
<br /> isk. The BIOSYS-1 program (Swofford and Selander isk.
<br /> 1981, 1989) was used to calculate Hardy-Weinberg and
<br /> FsT values. Sequential Bonferroni analyses were per- Population -
<br /> formed on statistical tests to adjust or account for Type I
<br />
<br />error rates (Rice 1989) Colorado Colorado
<br /> . Locus, River River ~
<br />
<br />Locus sample size, belpw above
<br /> ,
<br />sample alleles, and Moab, Cataract Moab, ~
<br />
<br />i mean FgT Utah Canyon Utah FgT ~
<br /> s
<br />ze, population
<br />alleles, * i
<br />ll
<br />EST-2 i i l
<br />
<br />and mean Colorado Green San Juan Yampa ~
<br />l
<br />N 29 7 2 0.032 ` j
<br /> FsT River River River River FgT *a 0.655 0.857 0.750 lj
<br /> EST-2* *b 0.310 0.143 0.250
<br />
<br />N 67 43 6 29 0.039 *c 0.034 0.000 0.000 ' !k~~i~
<br />GPI-1
<br /> ~
<br />0.701 0.733 0.817 0.759
<br />*6 ~ I~~
<br />N 30 7 2 0.013
<br /> 0.299 0.244 0.083 0.241
<br />*
<br />' *a 0.250 0.357 0.250 'i
<br /> ~
<br />0.000 0,023 0.000 0.000
<br />GPI-I * *~ 0.750 0.643 0.750
<br />a
<br />
<br />
<br />
<br />er
<br />N
<br />76* 49 11 33 0.004
<br />~
<br />GPI-2*
<br />i
<br />N 30 7 2 0.011
<br />~',li
<br />
<br />the 0.395 0.388 0.364 0.318
<br />*b 0.605 0.612 0
<br />636 0
<br />682 l
<br />~ 0.017 0.000 0.000 ~ ,
<br />
<br />ln- .
<br />.
<br />GPI-2* *6 0.983 1.000 1.OW
<br />
<br />N 76 49* I1 29 0.018
<br />
<br />* PEPS* I`
<br />1
<br />N 29 7 2 0.012 i
<br /> a 0.046 0.041 0.000 0.000
<br />*b 0.934 0.959 1
<br />000 0
<br />966 *a 0.983 1.000 1.000. I
<br />~
<br />
<br />'
<br />} .
<br />.
<br />~' 0.000 0.000 0.000 0.000 ~
<br />~ 0.017 0.000 0.000
<br />TPI-2*
<br />
<br />led *d 0.020. 0.000 0.000 0.034
<br />N 30 7 2 0.182 '~~
<br />ge_ PEPB* *a 0.000 0.000 0.250
<br />
<br />ire- N 64 49 10 33 0.009 *b L000 1.000 0.750
<br /> *a 0.008 0.000 0.000 0.000 PGAM-2*
<br />gum _ ~ 0.984 1.000 1,000 l.Opp N 30 7* 2 0.067
<br />Cur, *c 0.008 0.000 0.000 0.000 *a 0.033 0.143 0.000
<br />~~
<br />
<br />$1X PGAM-2* *b 0.967 0.857 1.000
<br />~
<br />
<br />
<br />files
<br />N 76* 49 11 33 0.027 Mean F ~ ~~
<br />sT 0.049
<br /> ~, 0.072 0.031 0.000 0.091 ~
<br />
<br />fre-
<br />*~ 0.928 0.969 1.000 0.909 ,
<br />]Tee Mean FgT 0.019 ~
<br />lions and low genetic differentiation. Eleven loci
<br />lele ~~
<br /> were polymorphic in samples of age-0 fish from
<br />'
<br />de- the Colorado and Green rivers when data from
<br />the six samples (P < 0.05). Uncommon alleles were Ammerman and Morizot (1989) were added (Table
<br />~
<br />P = found in both Colorado and Green River fish (EST- 7). Allele frequencies in age-0 Colorado pikemin- ~
<br />pies 2*c, GPI-2*a, GPI-2*d, and PEPB*c). The loci now agree closely with those obtained earlier b
<br />,~
<br />
<br />atia-
<br />PEPB* (Xz = 10.2, df = 1, P = 0.010) and PGAM- y
<br />.
<br />Ammerman and Morizot (1989; Table 7). The var- '' h
<br /> 2 (X - 44.4, df = 1, P = 0.001) in lower Green iability at TPI-I that was observed by Ammerman ;`'~ ~'
<br /> River samples and GPI-2* (xz = 64.3, df = 6, P and Morizot (1989) was not detected in the present ~
<br /> = 0.001) and PEPB* (xz = 14.5, df = 1, P = study, and variability at LDH-C*
<br />bGAL*
<br />and
<br />i fish 0.010) in lower Colorado River samples deviated ,
<br />,
<br />sMDH-2* was not assessed due to the lack of liver
<br />that significantly from H-W expectations. The mean samples from many individuals. Geographic var- ~~;
<br />Zilar, FgT of 0.046 suggests slight genetic differentiation iability of rare private alleles was found. The al-
<br />[-W at present. leles GR*b and TPI-2*c were found only in sam-
<br />t ge- We analyzed and compared these data with data ples from the Green River, and GPI-2*c
<br />PEPB*a
<br />
<br />loci
<br />previously collected on age-0 Colorado pikemin- ,
<br />,
<br />and PEPS*b in samples from the Colorado River
<br />~ ~ "
<br />
<br />from
<br />now. The presence of genetic variation,. similar .
<br />,
<br />The deviation from H-W equilibrium found in 1
<br />
<br />I. No
<br />gene frequencies, and uncommon alleles were con- , .l ,,
<br />Colorado and Green River age-0 fish (EST-2*) b
<br />
<br />g the
<br />firmed, as were deviations from H-W expecta- y
<br />Ammerman and Morizot (1989) was not verified ~~,.' I`
<br />~~~~ ~ l
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<br />~, !'
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