9424

• UCREFRP Catalog Number: 9424
• Author: Morizot, D. C., J. H. Williamson and G. J. Carmichael
• Title: Biochemical Genetics of Colorado Pikeminnow
• USFW Year: 2001
• USFW - Doc Type: North American Journal of fisheries Management
• Copyright Material: YES

_;.~,
--- - _ .
70
MORIZOT ETA

dates
-Site locations
BLE 3
T and numbers of fish (N) of 15 samples of adult, juvenile, and age-0 Colorado
n
i
;- ! ,
,
A
.
pikeminnow collected in 1990 to 1993 and analyzed for genetic variabili ty. See the captio n to Figur o
e 1 for an explanat
- i
of river kilometer measurements.
Sample
~ Site location River kilometers type N Date
Green River system
I~ ' 3-38.6
15 Adult 33 1992
Yampa River .
8-291.9
238
Adult
35 1992
Upper Green River .
2-86.9
54
Adult
14 1992
Lower Green River .
3-419.1
415 Age-0 101 1991

~'? Upper Green River .
9-224.3
216
Age-0
18 1990
_
~ Middle Green River .
8-86.9
69
Age-0
122 1990;1991
Lower Green River .
Colorado River system

Middle-upper Colorado River 177.5-294.9 Adult 51

25 1990-1991
1991-1992
i
- ~I Lower Colorado River
Ut
h
b
M 70.8-160.6
147.4-247.5 Adult
Juvenile
2 1991-1992-
~~~ a
oa
,
Colorado River above 7 Juvenile 30 1991-1992
Colorado River below Moab, Utah 26.6-120.
7 1991
Cataract Canyon -332.4 to -334.7 Juvenile
3 1991-1992
~ Middle-upper Colorado River 142.2-254.1
8
1 Age-0
e-0
A
169 1990-1991
~
?, Lower Colorado River .
12.4-9
7 to -340.3
-315 g
Age-0 13 1991
Cataract Canyon .
2
3-228
197
Adult
12 1991-1993
San Juan River .
.
_ `` ~ morphic protein-coding loci that were resolved are
i,
~,,: as follows (Enzyme Commission numbers
__ ~'i, [IUBMBNC 1992] are presented in parenthesis, with
the gene or protein symbol following each locus):
_..,~a;;.= ~ ~', esterase-2 (3.1.1.-; EST-2); glucose-6-phosphate
~I isomerase-1 (5.3.1.9; GPI-1); glucose-6-phosphate
isomerase-2 (5.3.1.9; GPI-2); glutathione reductase
(1.6.4.2; GR}; peptidase-S (3.4.11.1; PEPS); tripep-
tide aminopeptidase (3.4.11.4; PEPB); phosphoglyc-
I
!. erate mutase-2 (5.4.2.1; PGAM-2); and triose-phos-
phate isomerase-2 (5.3.1.1; TPI-2).
Wild Adults
Analyses of samples taken from wild adults in-
dicate that genetic variability is present, that gene
i`i' frequencies among populations are similar, that
' uncommon alleles and deviations from H-W equi-
librium exist, and that only low genetic divergence
is present among populations. Five of the eight
~~ polymorphic loci expressed in muscle or fin tissue
~~~ exhibited variability in spawning adult Colorado
pikeminnow (Table 4). Allele frequencies were not
significantly different among samples from the
four river basins (P < 0.05). Uncommon alleles
were shared by Colorado and Yampa River fish
(GPI-2*cl) and by Colorado and Green River fish
I,,l i
(GPI-2 *a). The allele EST-2 *c was restricted to
i ~i~i, the Green River population and PEPB*a and
PEPB*c to the Colorado river population. Signif-
',I~'II'.' icant deviations from H-W equilibrium expecta-
,i~~,
r~~iii' lions were observed at GPI-1 * (xZ = 10.4, df =
~~ 1, P = 0.001) and PGAM-2* (XZ = 8.4, df = 1, P
= 0.004) in Colorado River samples and at GPI-
2* (x2 = 64.7, df = 1, P = 0.001) in Green River
samples. The mean FsT value (0.019) among the
four populations of spawning adults suggests min-
imal genetic divergence (Wright 1978).
Juveniles
Analyses of samples from juveniles were limited
to the Colorado River but did show that some ge-
netic variability and one uncommon allele are pre-
sent, that one deviation from H-W equilibrium
exists, that no gene frequency differences occur,
and that slight genetic differentiation exists. Six
loci were polymorphic in samples of juveniles
from the Colorado River (Table 5). No allele fre-
quency differences were found among the three
samples (P < 0.05), and one uncommon allele
(GPI-2*a) was present. The locus PGAM-2* de-
viated significantly from H-W equilibrium in the
Cataract Canyon sample (XZ = 13.1, df = 1, P =
0.010). The mean FST among the three samples
(0.049) indicated only slight genetic differentia-
lion.
Age-0 Fish
Analyses of samples taken from wild age-0 fish
indicated that genetic variability is present, that
gene frequencies among populations are similar,
that uncommon alleles and deviations from H-W
equilibrium exist, and that there is only slight ge-
netic divergence among populations. Seven loci
were polymorphic in samples of age-0 fish from
the Colorado and the Green rivers (Table 6). No
allele frequency differences were found among the
TAE
tein-cc
popul~
nificar
Juan,
cant c
0.01) ;
isk. T
1981,
FST v
formes
error r:
Locus
sampL
size,
alleles
and me;
F'ST
EST-2*
N
*a
*b
*c
GPI-1
N
*~,
GPI-2*
N
*Q
*~
*d
PEPB*
N
*Q
*b
PGAM-2
N
*a
*b
Mean Fs~
six san
found i
2 *c, G.
PEPB*
2* (Xz
River s
= 0.00
0.010)
signific
FST of
at prese
We a
previou
now. T
gene fre
firmed,