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Nr . 1 •~-,. _~~ i8 is of ~y n- ia- :h- ter •ed :re ile ire ;tal on, lis- ors ~lo- ach Fro- and ge- uals rom an d at 991 Ales l fin; go- arch liza- midt iam- ~lved hism -ticu- d. on used 990). iabet- in or- ~e an- ~fford Itered ential istical adjust of the bution in al- :i con- BIOCHEMICAL GENETICS OF COLORADO PIKEMINNOW ,. -. 69 O FlovxE 1.-Colorado pikeminnow collection areas in the Colorado, Yampa, San Juan, and Green rivers. Numbers are river kilometers. Distances along the Green and San Juan rivers are measured from their confluences with the Colorado River, those along the Yampa River from its confluence with the Green River. Distances along the Colorado River above its confluence with the Green River are measured from that point and indicated by positive numbers; distances below the confluence (i.e., along Cataract Canyon) are indicated by negative numbers. taining two alleles, and chi-square goodness-of-fit tests were used to test for significant differences at loci containing three or more alleles, as de- scribed in Merrell (1975). Allozyme variability was analyzed separately for wild adults, juveniles, age-0 fish, and hatchery fish. Results Analyses of the biochemical genetics of Colorado pikeminnow in the wild and at the Dexter fish tech- nology center indicated that genetic variability is pre- sent. Eight loci were found to be polymorphic in at least one population sample using tris-EDTA-borate (pH 8.0) as a buffer. The use of minimally invasive sampling of fin and skeletal muscle tissue resulted in nonlethal sampling of wild adult and juvenile fish (N = 207) and hatchery-produced adults (N = 60) for all eight polymorphic loci. The hatchery-pro- duced fish were monitored- for several months and suffered no sampling-induced mortality. The poly-