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' he Instream Flow Incremental Methodology (IFlh11 was <br />developed by the U.S. Fish and Wildlife Service to pro- <br />vide a standard analytical technique for recommending <br />flows for a stream. Originally, IFiM was developed for <br />application in small simple cold-water stream systems from <br />v: hick water was to be diverted for off stream consumptive uses <br />or to be allocated for other development projects. One of the <br />initial objectives of the method was to assess with relative ease <br />changes in fish standing crop and species composition due to <br />changes in stream flow (Bovee 1978)..Several assumptions are <br />made in utilizing the method in a given stream. The purpose of <br />Our note is to critique some of these assumptions. <br />Only recently has the applicability of IFIM been investigated <br />in a relatively small wannwater stream (Orth and Maughan <br />1982). These authors provided sufficient data in the published <br />literature for our reanalysis. We will therefore use these data and <br />other published examples for the evaluation of the underlying <br />asssumptions. Undoubtedly. results of many other IFINI studies <br />exist in unpublished reports. nonrefereed papers, proceedings, <br />etc. <br />Theorn• and Afechanic•s of Calculations <br />An important integral component of IFIM is PIIABSIM <br />(Physical Habitat Simulation). The PHABSIM procedure con- <br />tains four primary components: (1) physical measurements of <br />depth, velocity, substrate., and cover within the stream reach, <br />(2) computer simulation of the stream hydraulics, (3) deter- <br />mination of a composite probability of use from the suitability <br />value for each combination of depth, velocity, and substrate <br />found within the stream reach, for each species and life history <br />phase, and (4) the calculation of weighted usable area (\1'UA) <br />for each stream flow, species, and life history phase for each <br />season. Other factors such as water quality and food can be <br />also incorporated in the calculation of WUA but the level of <br />complexity in application and interpretation increases substan- <br />tially. Biological interactions (competition, predation, etc.) are <br />recognized as important factors but at present cannot be <br />included in the application of the method. <br />The outputs of computer simulations of physical habitat <br />variables (based on measurements of depth, velocity, substrate, <br />cover, etc.) in a stream reach and species life stage "probability <br />of use" or "preference" or "suitability" curves (based on instan- <br />I,meous fish measurements in the field, expert opinion, or from <br />literature sources) are integrated into a potential available NVUA <br />for each flow. A relationship is then established between the <br />availability of potential WUA for each life history phase of a <br />species and stream flow in each season or time period. <br />Four basic assumptions are as follows: (1) depth, velocity, <br />and substrate are the most important physical habitat variables <br />affecting the distribution and abundance of fishes; b0im ioral <br />preference of a life stage of a species for each physical varinhie <br />can be established from instantaneous fish mcasurcnrcnrs in the <br />field and probability of use curves or suitability or utilii.alion <br />indices constructed; (2) depth, velocity, and substrate indepen- <br />dently influence habitat selection by fishes; (3) preference <br />factors for depth, velocity, and substrate, etc., can be combined <br />through multiplication to create WUA index; and (4) a positive <br />linear relationship exists between size of WUA and the biomass <br />of fishes: a slope of I is assumed to relate biomass and \VUA <br />(Bovee 1978). Presumably, an increase in the WUA will result <br />in increased fish populations because populations are implicitly <br />assumed to always be habitat limited. <br />926 <br />The WUA (in optimum habitat equi%alents). based on the <br />composite suitability of three principal habitat varia5les, is <br />derived for each stream reach from instantcureous measurement, <br />as follows: <br />WUA = C; A; <br />where Ci = f'(V,) x f(Di) . f; (S,), f,:(Vi) = nritabilitc <br />weighting factor for the selocit,. in cell i, f,i(D;) = suitabilil` <br />weighting factor for the depth in cell i. f (S,) = suitabilitd <br />weighting factor for the substrate type in cell i, )t = tile nunibe" <br />of cells, and A; = the area of cell i. Weighting factors are de <br />rived from "utilization" or "preference" or "suitability." "elec- <br />tivity," or "probability of use" curses. The methocl assumes thai <br />behavioral characteristics of a life stage of a species can by <br />defined by these tunes. The made or the peak of the curve i, <br />interpreted as the optimum value of a variable for fish usage [Intl <br />is given a weighting factor of 1. i-lie tails of the tune represent <br />zero weighting factor. Generall•:, weighting values bet•,veen t! <br />and I are empirically determined (in equid alent optimum habitat <br />units) from analysis of instantaneous observations of tish dis <br />tribution over the range of each variable (Bovee 1982). A.- <br />example of velocity and depth preference curve fur adult small <br />mouth bass..Sulnto&e.s dalcnniew. is given in Fig. I. <br />SuitabilitA- Irrdrx awl Prefrrcuc•t Cur vt.% <br />The original application of IFI(\i , treatment of "suitability" <br />"preference" cures as probability functions, led to the calculation of a joint probability function by multiplication of univari <br />ate preference factors as simple conditional probabilities (Bovee <br />1978, 1952). '1 his procedure is crrTect only when probabilitir <br />are statistically independent. A transformation of unitiariat, <br />preference factors into simple probabilities is erroneous. Fir,• <br />the mode or peak of curves 0i(mil in Fig. I only have <br />subjective rating of 1.0, chich i,, not cgtrivalent lo 'l I w+ahili, <br />of 1.0. That is, the curves shculd no! nugget ch,ct d ere i <br />100% chance (a certainty) of locating a species population <br />specified segment of a population. A rating of 1.0 -implj mean. <br />that most organisms were obsei vcd or captured at that deptl <br />and/or velocity at the time of collection. The cure does m?, <br />have any statistical distribution and cannot be considered as . <br />probability function. The ordinate values between 0 and I.u <br />(calculated from proportional scaling offish catches) hake beer <br />incorrectly interpreted a-z actual probahili!ics in i IIABSINI <br />Probability is an area under the cure and not a value of th, <br />ordinate. We are not aware of any published study that ha <br />addressed these statistical or mathematical distincti-ms. and }et <br />the suitability function in the form of the joint roil iihihl <br />function continues to be used (Bovee 1982). <br />The ratings of (lie "preference" or "suitabilit)' curves ar: <br />ratios. However, these ratios are haled upon a shifting denomin <br />ator. For example, if largest number, 10, were obtained a: <br />a particular depth and.or velocity , these variables ss ill be gis, <br />a rating (if 1.0. If in anolhcr sa!npling the )_rcatc,?t nun!her w„ <br />100 organisms the same yariahlc. would also he given a ratio; <br />of 1.0. Obviously. there is a ditfcrence in (he biomass of 10 all <br />100 organisms. In our view the development of the "preference' <br />curve as we described will lead one to expect loss correlati( <br />between "suitability" and fish standing stock. <br />Because fishes may respond to a multitude of factors in th <br />field, thus manifesting daily changes in their distribution <br />different tunes may be obtained on different sampling, dates o, <br />times within a season. Forexample, fishes change po.ition fr(ir <br />C.:v .1. f'r,A A.111111. S, i, ".1 . 42. 1Q <br />