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~~l~ E 729 (20) of aquatic animals is available in: Brauhn, J. L., and Schoettger, R. A., "Acquisition and Culture of Research Fish: Rainbow Trout, Fathead Minnows, Channel Catfish, and Bluegills," Ec- ological Research Series, EPA-660/3-75-011, U. S. Environmental Protection Agency, Corvallis, Ore., 1975, 54 pp.; Nutrient Requirements of Trout, Salmon, and Catfish, ISBN 0-309-02141- 3, 1973, 57 pp.; Aquatic Animal Health, ISBN 0- 309-02142-1, 1973,46 pp.; Fishes: Guidelinesfor the Breeding, Care and Management of Labo- ratory Animals, ISBN 0-309-02213-4, 1974, 85 pp.; Amphibians: Guidelines for the Breeding, Care and Management of Laboratory Animals, ISBN 0-309-0221O-X, 1974, 153 pp. National Academy of Sciences, Washington, D. c.; Spotte, S. H., Marine Aquarium Keeping, Wiley- Interscience, New York, 1970, 171 pp. Davis, J. c., and Hoos, R. A. W., "Use of Sodium Pentachlorophenate and Dehydroa- bietic Acid as Reference Toxicants for Salmonid Bioassays," Journal of the Fisheries Research Board of Canada, Vol 32, 1975, pp. 411-416; LaRoche, G., Eisler, R., and Tarzwell, C. M., "Bioassay Procedures for Oil and Oil Dispersant Toxicity Evaluation," Journal of the Water Pol- lution Control Federation, Vol 42, 1970, pp. 1982-1989; Adelman, I. R., and Smith, L. L., Jr., "Standard Test Fish Development, Part I," Ecological Research Series, EP A -600/3-76- 06Ia, U. S. Environmental Protection Agency, Duluth, Minn., 1976, 76 pp.; Fogels, A., and Sprague, J. B., "Comparative Short-Term Tol- erance of Zebrafish, Flagfish, and Rainbow Trout to Five Poisons Including Potential Ref- erence Toxicants," Water Research, Vol 11, 1977, pp. 811-817; Cardwell, R. D., Woelke, C. E., Carr, M. I., and Sanborn, E., "Appraisal of a Reference Toxicant for Estimating the Quality of Oyster Larvae," Bulletin of Environmental Contamination and Toxicology, Vol 18, 1977, pp.719-725. Hunn, J. B., Schoettger, R. A., and Whealdon, E. W., "Observations on the Handling and Maintenance of Bioassay Fish," Progressive Fish-Culturist, Vol 30, 1968, pp. 164-167. Butler, P. A., Wilson, A. J., and Rich, A. J., "Effect of Pesticides on Oysters," Proceedings of the Shellfish Association, Vol 51, 1960, pp. 23- 32; Environmental Research Laboratory-Gulf Breeze, "Bioassay Procedures for the Ocean Disposal Permit Program," EPA-600/9-76-01O. Gulf Breeze, Fla., 1976, pp. 81-83. Calabrese, A., Collier, R. S., Nelson, D. A., and MacInnes, J. R., "The Toxicity of Heavy Metals to Embryos of the American Oyster Crassostrea virginica," Marine Biology, Vol 18, 1973, pp. 162-166; Woelke, C. E., "Development of a Receiving Water Quality Bioassay Criterion Based on the 48-Hour Pacific Oyster (Crassos- trea gigas) Embryo," Technical Report No.9, Washington Dept. of Fisheries, Olympia, Wash., 1972,93 pp. For example, see: U.S. Environmental Protec- tion Agency, "Methods for Chemical Analysis (21) (22) (23) (24) 20 (25) of Water and Wastes," EPA-600-4-79-020, Cin- cinnati, Ohio, 1979, 460 pp.; Strickland, J. D. H., and Parsons, T. R., "A Practical Handbook of Seawater Analysis," Fisheries Research Board of Canada, Bulletin 167, Ottawa, 1968; National Handbook of Recommended Methodsfor Water- Data Aquisition, U.S. Dept. of the Interior, 1977; American Public Health Association, American Water Works Association, and Water Pollution Control Federation, Standard Methods for the Examination of Water and Wastewater, 14th ed., Washington, D.C., 1975, 1193 pp.; Association of Official Analytical Chemists, Official Meth- ods of Analysis, 12th ed., Washington, D.C., 1975, 1022 pp. Emerson, K., Russo, R. c., Lund, R. E., and Thurston, R. V., "Aqueous Ammonia Equilib- rium Calculations: Effect of pH and Tempera- ture," Journal of the Fisheries Research Board of Canada, Vol. 32, 1975, pp. 2379-2383; Bower, C. E., and Bidwell, J. P., "lonization of Am- monia in Seawater: Effects of Temperature, pH, and Salinity," Journal of the Fisheries Research Board of Canada, Vol. 35,1978, pp. 1012-1016. Litchfield, J. T., Jr., and Wilcoxon, F., "A Sim- plified Method of Evaluating Dose-Effect Ex- periments," Journal of Pharmacology and Ex- perimental Therapeutics, Vol. 96, 1949, pp. 99- 113; Finney, D. J., Statistical Method in Biolog- ical Assay, 2nd ed., Hafner Publishing Co., New York, 1964,668 pp.; Finney, D. J., Probit Anal- ysis, 3rd ed., Cambridge University Press, Lon- don, 1971, 333 pp.; Stephan, C. E., "Methods for Calculating an LC50," Aquatic Toxicology and Hazard Evaluation, F. L. Mayer and J. L. Hamelink, eds., ASTM STP 634, Am. Soc. Test- ing Mats., 1977, pp. 65-84. Draper, N. R., and Smith, H., Applied Regres- sion Analysis, Wiley, New York, 1966,407 pp.; Finney, D. J., 1964. Statistical Method in Bio- logical Assay, 2nd ed., Hafner Publishing Co., New York, 1964, 668 pp. Marking, L. L., and Dawson, V. K., 1973. "Tox- icity of Quinaldine Sulfate to Fish," Investiga- tions in Fish Control, No. 48, U. S. Fish and Wildlife Service, Washington, D.C., 1973,8 pp. Kester, D. R., Duedall, l. W., Connors, D. N., and Pytkowicz, R. M., "Preparation of Artificial Seawater," Limnology and Oceanography, Vol. 12, 1967, pp. 176-179; Zaroogian, G. E., Pesch, G., and Morrison, G., "Formulation of an Ar- tificial Sea Water Media Suitable for Oyster Larvae Development," American Zoologist, Vol. 9, 1969, p. 1144; Zillioux, E. J., Foulk, H. R., Prager, J. c., and Cardin, J. A., "Using Artemia to Assay Oil Dispersant Toxicities," Journal of the Water Pollution Control Federation, Vol. 45, 1973, pp. 2389-2396. Davey, E. W., Gentile, J. H., Erickson, S. J., and Betzer, P., "Removal of Trace Metals from Ma- rine Culture Media," Limnology and Oceanog- raphy, Vol. 15, 1970, pp. 486-488. Davis, H. S., 1953. Culture and Diseases of Game Fishes, University of California Press, Berkeley, 1953, 332 pp.; Hoffman, G. L., and Meyer, F. (26) (27) (28) (29) (30) (31)