7717

• UCREFRP Catalog Number: 7717
• Author: Rosenfeld, M. J.
• Title: Genetic Analysis of the Colorado River Gila
• USFW Year: n.d.
• USFW - Doc Type: Non-fatal Sampling, Cell Culture, Chromosomal Analysis and Electrophoresis.
• Copyright Material: NO

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tive study.

Subsequent to obtaining th~ cytogenetic and electrd-

phoretic samples~

fish were

f i :{ ed

in

10% borate-buffer.d

,
formalin for deposition in the author"s collection~ with Carliln

tag numbers as follows:

30194~

3020(l~

3()236,

30240 ~ 302411,

3()24.3~

30247~

30249.

Ten bony tail

chubs~

the 3+ yearclass

progeny of brood stock caught at Lake Mojave and kept at the

Dexter National Fish Hatchery~ New Mexico~ were sampled~ fixed jn

10% borate-buffered formalin and stored as ELI

EL10 in the

author's collection.

Four hybrid ~l!_~_ derived from crossEf's

involving roundtail~ bony tail and humpback chubs were provided py

the Dexter National Fish Hatchery.

The fish were identified py

fin clips <i.e., missing fins) as follows:

Their parentag~s

were not given to the author; rather, determination of whi~h

species comprised the hybrid genome were derived in this study

and subsequently checked.

Twenty Utah chubs, ~. atraria, frpm

Timpie Springs, Utah,

were processed

in the same manner and

numbered as T51-T520 in the author"s collection.

The 1 attier

species was utilyzed as an outgroup to provide a basis fior

inferring phylogenetic relationships among the Colorado Ri~er

8i 1 a.

CytoQenetics:

Whether in the fIeld or

laboratory~ asep~ic

,
procedures were not followed when obtaining fIn tIssues, altho~gh

forceps and scissors were cleansed with 70% ethanol

prior to