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<br />two dJtferent migration dIstances wh~n extracted trom two fISh, <br /> <br />then those individuals would not posess the same gene seque~ce <br /> <br />coding for that protein and would be genetically-different. <br /> <br />Electrophoresis has been used for stock determination in fish~s, <br /> <br />especially among the salmonids <br /> <br />(Utter et al. ~ <br /> <br />19T:;~ All endqrf <br /> <br />et al., 1977) . I nasi tuat ion si mi 1 ar to the sympatry recorqed <br /> <br />for Gila sp. in the Lipper Colorado River, genetic isolatiion <br /> <br />as well as intergradation in darters of the Ozark Uplands h~ve <br /> <br />been convincingly demonstrated using this technique (Wiseman let <br /> <br />al., 1978). The data in most electrophoretic studies are in ~he <br /> <br />form of differences in the frequency of allozymes, which enta~ls <br /> <br />large sample sizes and usually the sacrifice of specimens. T~is <br /> <br />protocol obviously cannot be part of an identification prog~am <br /> <br />involving endangered fishes, most especially since it is often <br /> <br />necessary to identify only one or a few individuals. Itiis <br /> <br />also desirable, if not requisite, that identification pro~e- <br /> <br />dures not entail the sacrifice of animals. <br /> <br />MATERIALS AND METHODS <br /> <br />Samplinq: Humpback and roundtail chubs were caught with <br /> <br />46.2 X 1.8 m trammel nets at Blackrocks Canyon, Colorado, 217.6 - <br /> <br />219.2 km from the confluence of the Green and Colorado Rive}s. <br /> <br />BecausE.> of their legal statu~:;. as; well -as curr'ent use in tr-ack:\.ng <br /> <br />studies, humpback chubs were <br /> <br />not sacrificed. <br /> <br />Instead, samPtes <br /> <br />narcotized w~th <br /> <br />were taken, <br /> <br />below, from fish <br /> <br />as described <br /> <br />t ,.. ] L~, j n", m Co t h d ri D S U 1 P t-j d t c" . <br /> <br />{~l] Sr'E'C j mE'f.,':' r pcc'j V.E'C! Car-]I r. tdgS -rnd <br /> <br /> <br />F. <br />