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Last modified
7/14/2009 5:02:30 PM
Creation date
5/20/2009 3:04:31 PM
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UCREFRP
UCREFRP Catalog Number
7717
Author
Rosenfeld, M. J.
Title
Genetic Analysis of the Colorado River Gila
USFW Year
n.d.
USFW - Doc Type
Non-fatal Sampling, Cell Culture, Chromosomal Analysis and Electrophoresis.
Copyright Material
NO
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<br />"-- . , f-' .. ~.. <br /> <br />we gain a more complete understanding of the ge~etic structureiof <br /> <br />the Colorado Ri ver Gi I a compl e>:. <br /> <br />Ultimately we must be able to <br /> <br />identify individuals from different gene pools. <br /> <br />Since species <br /> <br />recovery entails strategies to increase population size~ it is <br /> <br />important to <br /> <br />understand genetic variation within surviv~ng <br /> <br />populations in order to also preserve diversity (Beardmo~e, <br /> <br />1983) . <br /> <br />Investigations at the cytogenetic and electrophoretic lev~ls <br /> <br />of resolution have not been previously tried with respect! to <br /> <br />identifying Gila from the Colorado River; this report is ~he <br /> <br />first to attempt it from those <br /> <br />perspectives. <br /> <br />Unambigupus <br /> <br />identifications could be possible if populations or species w.ere <br /> <br />characterizable by <br /> <br />distinct chromosomal <br /> <br />states or pro~ein <br /> <br />electromorphs. <br /> <br />Cytogenetics entails analyses of the morphology and interinal <br /> <br />details of chromosomes (see Ojima, 1983, <br /> <br />for a review speci!fic <br />I <br /> <br />to fishes). <br /> <br />Chromosomes contain the genetic material and :our <br /> <br />understanding of chromosomes with respect <br /> <br />to the reproduc~ive <br />I <br /> <br />process is <br /> <br />fairly substantial; <br /> <br />consequently, <br /> <br />one might be <br /> <br />able to not only distinguish between individuals but also pre~ict <br /> <br />the consequences of hybridization on the basis of chromos~mal <br /> <br />differences. <br /> <br />Electrophoresis entails the mo~ement of <br /> <br />protei ns in an <br /> <br />electric field~ <br /> <br />with different proteins migrating diffe~ent <br /> <br />distances. <br /> <br />If a protein (e.g., an enzyme such as malate dehy~ro- <br />
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