Laserfiche WebLink
<br />3 <br />in the Colorado squawfish holding tanks. Colorado squawfish seemed to lack <br />sufficient motivation to feed when tested singularly (trials A-C, Appendix) <br />and were therefore tested in groups of three. They also appeared to lack <br />interest in channel catfish as prey items (trials D-G, Appendix); hence, only <br />those fish that would eat catfish with their spines removed (CCSPL) were <br />exposed to catfish with their spines intact (CCSP). The experimental pro- <br />tocol was as follows: The Colorado squawfish to be used in a particular <br />feeding trial were starved for 5 days before the trial. On the morning of <br />the trial they were transferred to the experimental aquarium and allowed to <br />recover from handling stress and acclimate for 6-7 hr. The 40 catfish to <br />be used in the feeding trial were tranquilized with tricane methane sulfonate <br />and quinaldine and measured. On the morning of the trial dorsal and pectoral <br />spines were clipped and fish were placed in a recovery aquarium for 5-6 hr. <br />This method of spine removal evidently has no apparent effect on the prey <br />(Reist, 1980). The CCSPL were released in the experimental aquarium with the <br />Colorado squawfish in the late afternoon at a time when squawfish are nor- <br />mally fed. They were observed for the first 0.5 hr and then left with the <br />squawfish for 90 hr. The catfish were fed during this period, but not the <br />Colorado squawfish. On the morning of the fifth day the Colorado squawfish <br />were removed from the aquarium, after which the catfish were removed and <br />counted. If any had been eaten, the remaining catfish were tranquilized, <br />remeasured, and then returned to the recovery aquarium. Colorado squawfish <br />used in the trial were placed back in experimental aquarium and left alone <br />until the afternoon. If any CCSPL had been eaten, then 40 catfish with <br />spines intact (CCSP) were placed in the aquarium. Fish were observed for <br />0.5 hr after introduction and left together for another 90 hr. After feeding