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3. Whole Effluent Toxicity Testing Requirements - Chronic WET Testing - Outfall(s): 004X <br />(a) Testing and Reporting Requirements <br />(b) <br />(c) Automatic Compliance Schedule Upon Failure of Test <br />(i) proceed to conduct the PTI/TIE investigation as described in Part I.A.4.e, or <br />(ii) conduct accelerated testing using the single species found to be more sensitive. <br />(e) PTI/TIE <br />trt I <br />Page No. 4 <br />Permit No.: CO- 0027529 <br />Tests shall be done at the frequency listed in Part I.B.1. Test results shall be reported along with the Discharge <br />Monitoring Report (DMR) submitted for the reporting period during which the sample was taken. (i.e., WET testing <br />results for the first calendar quarter ending March 31 shall be reported with the DMR due April 28.) The results shall be <br />submitted on the Chronic Toxicity Test report form, available from the Division. Copies of these reports are to be <br />submitted to both the Division and EPA along with the DMR. <br />The permittee shall conduct each chronic WET test in general accordance with methods described Short Term Methods <br />for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA/600 /4- 89/001 or <br />the most current edition, except as modified by the most current Division guidance document entitled Guidelines for <br />Conducting Whole Effluent Toxicity Tests. The permittee shall conduct such tests using Ceriodapluda dubia and fathead <br />minnows. <br />Failure of Test and Division Notification (Beginning on Effective Date of Limitation) <br />Beginning on the effective date of the limitation, a chronic WET test is failed whenever there is a statistically significant <br />difference in lethality between the control and any effluent concentration less than or equal to the instream waste <br />concentration ( "IWC "). The IWC for this permit has been determined to be 10.3 %. The permittee must provide written <br />notification of the failure of a WET test to the Division, along with a statement as to whether a Preliminary Toxicity <br />Investigation ( "PTI ")/Toxicity Identification Evaluation ( "TIE ") or accelerated testing is being performed. Notification <br />must be received by the Division within 21 calendar days of the demonstration of chronic WET in the routine <br />required test. "Demonstration" for the purposes of Parts I.A.4(b),(c),(d), (e) and (g) means no later than the last day of <br />the laboratory test. <br />If a routine chronic WET test is failed, the following automatic compliance schedule shall apply. As part of this the <br />permittee shall either: <br />If accelerated testing is being performed, the permittee shall provide written notification of the results within 14 <br />calendar days of completion of the "Pattern of Toxicity " / "No Toxicity" demonstration. Testing will be at least <br />once every two weeks for up to five tests until; 1) two consecutive tests fail or three of five tests fail, in which case a <br />pattern of toxicity has been demonstrated or 2) two consecutive tests pass or three of five tests pass, in which case no <br />pattern of toxicity has been found. If no pattern of toxicity is found the toxicity episode is considered to be ended and <br />routine testing is to resume. If a pattern of toxicity is found, a PTI/TIE investigation is to be performed. If a pattern of <br />toxicity is not demonstrated but a significant level of erratic toxicity is found, the Division may require an increased <br />frequency of routine monitoring or some other modified approach. <br />The results of the PTI/TIE investigation are to be received by the Division within 120 days of the demonstration <br />of chronic WET in the routine test, as defined above, or if accelerated testing is performed, the date the pattern of <br />toxicity is demonstrated. A status report is to be provided to the Division at the 30,60 and 90 day points of the <br />PTI/TIE investigation. The Division may extend the time frame for investigation where reasonable justification exists. <br />A request for an extension must be made in writing and received prior to the 120 day deadline. Such request must <br />include a justification and supporting data for such an extension. <br />