2012-06-28_PERMIT FILE - M2012032 ‎(18)‎

• Permit No: M2012032
• IBM Index Class Name: PERMIT FILE
• Doc Date: 6/28/2012
• Doc Name: 112D‎(1)‎ APPLICATION PACKAGE, APPENDIX 3, PART 1 OF 2
• From: STAR MINE OPERATIONS
• To: DRMS
• Email Name: GRM
• Email Name: RCO
• Media Type: D
• Archive: No

Test organisms were water fleas (Ceriodaphnia dubia) obtained from the ENSR /FCETL in-
house culture (FCETL batch #061697). At test initiation, the test organisms were <24
hours old (all within 8 hours of the same age) and appeared to be in good physical
condition. Test organisms were transferred directly from culture water (at test temperature)
into test solutions without additional acclimation.
The most recent chronic reference toxicant test using Ceriodaphnia dubia from the ENSR in-
house culture was initiated June 3, 1997. Sodium chloride was used as the reference
toxicant with moderately hard reconstituted water as the dilution water; the test method
was USEPA method 1002.0.
7.1 Biological Data
5.0 TEST ORGANISMS
6.0 QUALITY ASSURANCE
7.0 RESULTS
8503 -096 -193 -008
Daily survival and mean reproduction of test organisms are provided in the following table.
'One test organism was not found and was considered a mortality.
6
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0
(Perform.
Control)
100
100
100
90
80
80
SO
80
22.0
N/A
N/A
0
(Control
90
90
90
90
90
90
90
90
24.0
N/A
N/A
25
100
100
100
100
100
100.
100
100
24.3
NO
NO
35
100
100
100
100
100
100
100
100
28.4
NO
NO
49
100
100
100
100
100
90'
80
80
18.8
NO
NO
75
100
100
100
100
100
100
100
100
21.8
NO
NO
100
100
100
100
100
100
80
90
90
20.5
NO
NO
Test organisms were water fleas (Ceriodaphnia dubia) obtained from the ENSR /FCETL in-
house culture (FCETL batch #061697). At test initiation, the test organisms were <24
hours old (all within 8 hours of the same age) and appeared to be in good physical
condition. Test organisms were transferred directly from culture water (at test temperature)
into test solutions without additional acclimation.
The most recent chronic reference toxicant test using Ceriodaphnia dubia from the ENSR in-
house culture was initiated June 3, 1997. Sodium chloride was used as the reference
toxicant with moderately hard reconstituted water as the dilution water; the test method
was USEPA method 1002.0.
7.1 Biological Data
5.0 TEST ORGANISMS
6.0 QUALITY ASSURANCE
7.0 RESULTS
8503 -096 -193 -008
Daily survival and mean reproduction of test organisms are provided in the following table.
'One test organism was not found and was considered a mortality.
6