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3.2 Dilution /Control Water <br />The dilution water was reconstituted laboratory water prepared to match (t 15%) the <br />hardness of the receiving water (40 mg/t. as CaCO - ENSR sample #10042). A <br />performance control was conducted concurrently using moderately hard reconstituted water <br />prepared according to USEPA (1994) guidelines. initial characterization of the dilution water <br />and performance control water was as follows: <br />2640 (Performance <br />Control Water) <br />94 64 <br />2648 (Dilution Water) 38 31 <br />151 <br /><1. <br /><1.0 <0.05 <br />As CaCO <br />'Total Residual Chlorine. <br />4.1 Test Method <br />4.0 TEST CONDITIONS <br />8503 - 096 - 193 -008 <br />The study was a static - renewal short-term chronic test using Ceriodaphnia dubia, and was <br />conducted based on USEPA (1994) and Colorado Department of Health (1993) guidelines. <br />The biological responses measured were death (defined as no visible movement nor any <br />response to gentle prodding with a blunt probe) and young production. The complete test <br />protocol is included as Appendix B. <br />4.2 Test Duration <br />Test duration was 8 days, beginning 1630 June 17, 1997 and ending 0925 June 25, 1997. <br />4.3 Test Apparatus <br />Test chambers were 30-ml plastic beakers containing 15 ml of test solution. Ten replicate <br />chambers were tested for each treatment, with 1 organism per chamber (10 per treatment). <br />The test chambers were held in an environmental chamber under fluorescent lighting with a <br />photoperiod of 16 hours light: 8 hours dark; target test temperature was 25 t 1 °C. <br />4.4 Feeding <br />Test organisms were fed 0.2 ml of a 1:1 mixture of algae (Selenastrum capricornutum) <br />suspension (3.0 X 10' - 3.5 X 10' cells/ml) and an incubated mixture of yeast, trout chow, <br />and cereal leaves_(YTC; USEPA 1994) daily during testing. <br />4.5 Aeration <br />Test chambers were not aerated during this study. <br />5 <br />