2012-06-28_PERMIT FILE - M2012032 ‎(18)‎

• Permit No: M2012032
• IBM Index Class Name: PERMIT FILE
• Doc Date: 6/28/2012
• Doc Name: 112D‎(1)‎ APPLICATION PACKAGE, APPENDIX 3, PART 1 OF 2
• From: STAR MINE OPERATIONS
• To: DRMS
• Email Name: GRM
• Email Name: RCO
• Media Type: D
• Archive: No

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2691 (Performance
Control Water)
96
66
336
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2696 (Dilution Water
40
23
142
<1.0
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3.2 Dilution /Control Water
The dilution water was reconstituted laboratory water prepared to match (t 15 %) the
hardness of the receiving water (38 mg /L as CaCO ENSR sample #10165). A
performance control was conducted concurrently using moderately hard reconstituted
water prepared according to USEPA (1994) guidelines. Initial characterization of the
dilution water and performance control water was as follows:
'As CaCO
'Total Residual Chlorine.
4.1 Test Method
4.0 TEST CONDITIONS
8503 - 099 - 193 -010
The study was a static - renewal short -term chronic test using Ceriodaphnia dubia, and was
conducted based on USEPA (1994) and Colorado Department of Health (1 993) guidelines.
The biological responses measured were death (defined as no visible movement nor any
response to gentle prodding with a blunt probe) and young production. The complete test
protocol is included as Appendix B.
4.2 Test Duration
Test duration was 6 days, beginning 1445 July 29, 1997 and ending 1510 August 4,
1997.
4.3 Test Apparatus
Test chambers were 30 -m1 plastic beakers containing 15 ml of test solution. Ten replicate
chambers were tested for each treatment, with 1 organism per chamber (10 per
treatment). The test chambers were held in an environmental chamber under fluorescent
lighting with a photoperiod of 16 hours light: 8 hours dark; target test temperature was
2511 °C.
4.4 Feeding
Test organisms were fed 0.2 ml of a 1:1 mixture of algae (Selenastrum capricornutum)
suspension (3.0 X 10' - 3.5 X 10 cells /ml) and an incubated mixture of yeast, trout chow,
and cereal leaves (YTC; USEPA 1994) daily during testing.
5