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Concentration <br />No. <br />Alive <br />33 <br />38 <br />39 <br />37 <br />32 <br />37 <br />Avg. Wt. <br />(mg) <br />0.218 <br />0.238 <br />0.220 <br />0.235 <br />0.216 <br />0.245 <br />Min. <br />0.187 <br />0.208 <br />0.164 <br />0.197 <br />0.196 <br />0.241 <br />Max. <br />0.238 <br />0.258 <br />0.239 <br />0.275 <br />0.240 <br />0.252 <br />_; . , :. <br />Ifelre> -: <br />-_ . °;ir'o <br />Lower <br />bound <br />Control (0 %) <br />12.5% <br />25% <br />50% <br />75% <br />100% <br />Lower <br />bound <br />Upper <br />bound <br />pa et rs: <br />d i tc5 <br />e' <br />ifn ib <br />=. <br />iiinits foie <br />the #est." <br />N/A <br />N/A <br />12 <br />30 <br />13 <br />PMSD <br />(% Minimum <br />significant <br />difference) <br />Survival <br />Growth <br />Reproduction <br />Ceriodaphnia dubia <br />Fathead <br />Minnow <br />Lower <br />bound <br />Upper <br />bound <br />Lower <br />bound <br />Upper <br />bound <br />pa et rs: <br />d i tc5 <br />e' <br />ifn ib <br />=. <br />iiinits foie <br />the #est." <br />N/A <br />N/A <br />12 <br />30 <br />13 <br />47 <br />20.6 <br />31.3 <br />Client: Western Fuels <br />Site: New Horizon Mine 007 <br />CO- 0000213 SCG Project No.: 311693.B <br />Fathead minnow Test Results <br />Fathead minnow results are summarized in Table 3 and are provided on data sheets in <br />Appendix 3. Survival was 93% in the 100% effluent concentration and ranged from 80 %- 98% <br />in the remaining effluent concentrations. Control survival was 83%. No statistically significant <br />mortality was measured in any effluent concentration when compared to the control. The NOEC <br />for lethality was 100 %. The IC2 for lethality was >100%. <br />Table 3. Summary of Fathead minnow test results. Forty fish were exposed at each <br />concentration. An asterisk ( *) denotes a statistically significant difference from the control. <br />Average weight in the 100% effluent concentration was 0.245mg per individual and <br />ranged from 0.216mg - 0.238mg per individual in the remaining effluent concentrations. <br />Average weight for the control fish was 0.218mg for statistical analyses and 0.268mg for test <br />acceptability criteria. No statistically significant differences were measured for growth in any <br />effluent concentration when compared to the control. The NOEC for growth was 100 %. The IC <br />for growth was >100%. <br />Test Acceptability <br />Acceptable control survival was achieved in both tests. Similarly, Ceriodaphnia dubia <br />reproduction and Fathead minnow growth in control organisms met required levels. PMSD was <br />within the required limits for an acceptable test (Table 4). <br />Table 4. PMSD for chronic test parameters. <br />SeaCrest Group <br />8 <br />