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Concentration <br />No <br />Surviving <br />Mean <br />Births <br />Min. <br />Max. <br />ca <br />5 <br />=: ii'0 <br />Control (0 %) <br />10 <br />32.1 <br />18 <br />40 <br />Reproduction IC <br />Ceriodaphnia dubia <br />20% <br />10 <br />34.5 <br />27 <br />43 <br />N/A <br />Dunnett's Test <br />40% <br />9 <br />27.0 <br />0 <br />34 <br />60% <br />9 <br />25.2 <br />0 <br />35 <br />80% <br />7 <br />22.1 <br />0 <br />37 <br />100% <br />8 <br />22.9 <br />13 <br />35 <br />Species <br />Normality <br />Homogeneity <br />Ceriodaphnia dubia <br />Kolmogorov Test <br />Bartlett's Test <br />Fathead minnow <br />Shapiro — Wilk's Test <br />Bartlett's Test <br />Statistical Difference <br />Survival <br />Growth <br />Reproduction IC <br />Ceriodaphnia dubia <br />Fisher's Exact Test <br />N/A <br />Dunnett's Test <br />ICp <br />Fathead minnow <br />N/A <br />Dunnett's Test <br />N/A <br />ICp <br />Client: Western Fuels <br />Site: New Horizon Mine 007 <br />CO- 0000213 <br />RESULTS <br />SCG Project No.: 311693.B <br />Table 1. Statistics methods used in testing for sgnificant differences in test parameters. <br />Ceriodaphnia dubia Test Results <br />Test results for the Ceriodaphnia dubia are summarized in Table 2 and provided on the <br />data sheets located in Appendix 2. Survival was 80% in the 100% effluent concentration and <br />ranged from 70% - 100% in the remaining effluent concentrations. Control survival was 100 %. <br />No statistically significant mortality was measured in any effluent concentration when compared <br />to the control. The No Observable Effect Concentration (NOEC) for lethality was 100 %. The <br />IC for lethality was >100 %. <br />Table 2. Summary of Ceriodaphnia dubia test results. Ten animals were exposed at each <br />concentration. An asterisk ( *) denotes a statisticall y significant difference from the control. <br />Average numbers of neonates was 22.9 in the 100% effluent concentration and ranged <br />from 22.1 - 34.5 in the remaining effluent concentrations. Average number of neonates in the <br />control was 32.1 for statistical analysis and for test acceptability criteria. No statistically <br />significant differences in the number of neonates were found between the control and any <br />effluent concentration. The NOEC for reproduction was 100 %. The IC/ for reproduction was <br />61.7 %. <br />SeaCrest Group <br />7 <br />