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COLORADO - CDPS WET TEST REPORT FORM - CHRONIC <br />Permitee: Twentymile Coal Company - Site 5D <br />CDPS Number: CO - 034142 <br />Outfall: Site 5D 2nd Quarter 2010 <br />Type of Test: Routine-Annual Lot #: 061510-2abc <br />Test Species: Pimephales promelas <br />Test Results Survival Growth Statistical Method <br />IC25 Point Estimate >100% >100% <br />Pass/Fail PASS NA Linear Interpolation <br />Concentration with Least Statistical Difference >100% 100% Steel's Many-One Rank <br />Pass/Fail PASS NA Steel's Many-One Rank <br />Pimephales promelas Daily Percent Survival (%) <br /> Control 12.5% 25% 50% 75% 100% <br />Initial ! <br />100 100 100 <br />J <br />Day1 100 100 100 <br />Day 2 100 100 W 100 <br />Day 3 100 100 100 <br />Day 4 100 100 100 <br /> 100 100 inn 100 <br />Day 6 100 100 100 100 100 100 <br />_ Day 7 100 100 100 100 100 100 <br />_ 47- <br />7. daymean dry weight (mg) 4.471 0.463 0.468 0.456 0.444 0.430 <br /> <br />pH (minimum/maximum) 7.7/8.3 7.7/8.4 7.6/8.5T 7.6/865 7.6/8.6 7.5/8.7 5 <br />Receiving Water used for dilution? NO Total Residual Chlorine: ND <br />Hardness LM-9/0 Rec Water NA Effluent 1 365;73 ?, MHFSW 96:99 <br />Alkalinity mg/L) Rec Water NA ; Effluent :722,`63 - MHFSW 57:81. <br />Ammonia (mg/L) Rec Water .,,`-'°NA ,._ Effluent ND;`. z MHFSW ,?' ,ND <br />Were all test conditions in conformance with division guidelines? YES <br />If "NO", list deviations from test specification: NA <br />Laboratory: Aqua-To. <br />Analyst(s):