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COLORADO - CDPS WET TEST REPORT FORM - CHRONIC <br />Permitee: Twentymile Coal Company - Site 5D <br />CDPS Number: CO-034142 <br />Outfall: Site 5D 2nd Quarter 2010 <br />Type of Test: Routine-Annual Lot #: 061510-2abc <br />Test Species: Ceriodaphnia dubia <br />Test Results Survival Reproduction Statistical Method <br />IC25 Point Estimate >100% 98.43% <br /> <br />Pass/Fail <br />PASS <br />NA Linear Interpolation <br />Concentration with Least Statistical Difference >100% 100% Fisher's Exact Test <br />Pass/Fail PASS NA Dunnett's Test <br />Ceriodaphnia dubia Daily Percent Survival (%) <br /> Control 12.5% 25% 50% 75% 100% <br />Initial 100 100 100 100 100 100 <br />Day1 100 100 100 100 100 100 <br />Day 2 100 100 100 100 100 100 <br />Day 3 100 100 100 100 100 100 <br />Day 4 100 100 100 100 100 100 <br />Day 5 100 100 100 100 100 100 <br />Day 6 100 100 100 100 100 100 <br />Day 7 100 100 100 100 100 100 <br /> <br />Mean 3 Brood Total 34.8 35.1 34.5 33.8 33.9 25.7 <br /> <br />H minimum/maximum <br />p ( ) 8.0/8.3 7.9/8.4 7.8/8.6 7.7/8.7 7.6/8. <br />8 <br />7.5/8.9 <br />Receiving Water used for dilution? NO Total Residual Chlorine: ND <br />Hardness (mg/L) Rec Water Effluent 6.7 3* MHFSW =96.99 <br />Alkalinity (mg/L) Rec Water Effluent 722:63 ` MHFSW 57:81. <br />Ammonia (mg/L) Rec Water Effluent MHFSW ND " <br />Were all test conditions in conformance with division guidelines? YES <br />If "NO", list deviations from test specification: NA <br />Laboratory: Aqua-Tox, <br />Analyst(s):