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Table 1 - Colorado Chronic Test Conditions <br />Parameter <br />Ceriodaphnia dubia <br />Fathead Minnow <br />Test Type <br />Temperature <br />Photoperiod <br />Test Chamber Size <br />Test Sample Volume <br />Renewal Period <br />Age of Organisms <br />Number per Chamber <br />Chambers per Concentration <br />Feeding Regime per Chamber <br />Test Duration <br />Static renewal <br />25+/-1°C <br />16 hr light/8 hr dark <br />30 mL <br />20 mL <br />24 hrs <br />< 24 hrs <br />1 <br />10 <br />0.1 ml Algae/YTC per day <br />Until 60% of control organisms <br />have 3 broods or 8 days <br />Table 2 - Methods and Equipment <br />Static renewal <br />25 +/- 10C <br />16 hr light/8 hr dark <br />600 mL <br />300 mL <br />24 hrs <br />< 24 hrs <br />10 <br />4 <br />0.15 ml Brine shrimp 2x/day <br />7 days <br />Characteristic Method/Equipment Method <br />Alkalinity Titrimetric SM 2320 <br />Ammonia CHEMetrics Colorimetery K-1510 <br />Chlorine, Total Residual CHEMetrics Colorimetery K-2504 <br />Conductivity Orion Model 160 Conductivity Meter SM 2510 <br />Dissolved Oxygen YSI Model 59 DO Meter EPA 360.1 <br />Hardness Titrimetric SM 2340 B & EPA 200.7 <br />pH Titrimetric SM 4500 H <br />Temperature NIST5 calibrated thermometer <br />Analytical Balance Mettler AE 240 <br />References <br />Methods for Chemical Analysis of Water and Waste, Environmental Protection Agency, Environmental <br />Monitoring Systems Laboratory - Cincinnati (EMSL-CI), EPA-600/4-79-020, Revised March 1983 <br />Colorado Department of Health 1998 Guidelines for Conducting Whole Effluent Toxicity Tests Water Quality <br />Control Division, Denver, Colorado 80246-1530. <br />US EPA, October 2002, Short-Term Methods for Estimating the Chronic Toxicity of Effluents and <br />Receiving Waters to Freshwater Organisms, Fourth Edition, U.S. Environmental Protection Agency, <br />Office of Water, Washington, D.C. EPA-821-R-02-013 <br />5 National Institute of Standards and Technology