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Narrative and Discussion <br />• PERMIT AND SAMPLE INFORMATION <br />NPDES permit #: <br />Type of sample: <br />Date and time of sample(s): <br />Date and time of test start: <br />Dilution water: <br />Receiving temperature(s): <br />• TEST ORGANISMS <br />Fathead minnow <br />Ceriodaphnia dubia <br />• PROCEDURE <br />CO-0000213 <br />Effluent Composite <br />09/15/08 11:15, 09/17/0810:50 <br />09/16/08 11:30 <br />Reconstituted Laboratory Water <br />10.5°C, 6.0°C <br />Age: <24 hrs <br />Age: 6-13 hrs <br />Chronic bioassays as outlined in the most recent protocols specified by the Water Quality Control <br />Division of the Colorado Department of Health (1998). <br />• DISCUSSION <br />According to the Western Fuels New Horizon Mine discharge permit (CO-0000213), a chronic <br />WET test is failed whenever there is a statistically significant difference in lethality between the <br />control and any effluent concentration less than or equal to the Instream Waste Concentration. <br />The IWC for this permit has been determined to be 100%. <br />By this definition, the test results indicate that the effluent was not chronically toxic to <br />fathead minnow or to Ceriodaphnia dubia survival. Therefore, outfall 007 is in compliance <br />with the chronic WET portion of its Colorado State Discharge Permit for the third quarter <br />of 2008. <br />DATE OF REPORT <br />September 25, 2008 <br />reviewed by: Stephan Green Dawkins <br />44? I <br />t's <br />Isor Bio ssay Laboratory Date <br />COMMENTS: Initial sample receiving temperature was 10.5°C.' Client was contacted and consented to test <br />initiation.