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I Introduction <br />Water sampling from lakes has been conducted through out the United States. Although <br />water sampling from lakes has been performed in Colorado, the Colorado Water Quality <br />Control Division does not have a recommended protocol for obtaining samples from <br />lakes. The Water Quality Division at the Texas Natural Resource Conservation <br />Commission (TNRCC) does have a protocol for sampling organics in the Surface Water <br />Quality Monitoring Procedures Manual. The TNRCC sampling information can be <br />viewed at http://www.tnrcc.state.tx.us/admin/topdoc/gi/252.html. This protocol for <br />sampling lakes is based upon the TNRCC protocols. <br />2 Project Description <br />This project is designed to take a sample of an irrigation well and have the sample <br />analyzed for DIMP (diisopropyl methylphosphonate). There is little information <br />currently available on the toxicity and characteristics of DIMP1-d 2. Based upon the <br />available information it is not clear that DR\4P is a likely found at the top or the bottom of <br />the lake being sampled. A water solubility value of 1500 mg/L is estimated for DIlVIP3 <br />and based upon this value and the polarity of DIMP it regarded as soluble. For many <br />years the Tri-County Health Department (TCHD) has been sampling water wells and <br />bodies of water for DIMP. <br />The lake to be sampled for this project is the Jeronimus Lake located south of 104th <br />Avenue and East of Fulton Ditch in Adams County, Colorado. The Jeronimus Lake will <br />be dewatered for mining activities and water will be sampled to determine the absence of <br />DIMP or the concentration of DIMP in the lake. <br />The sampling protocol is written for sampling the lake. This sampling protocol will be <br />used to obtain the water sample and submit the sample to the laboratory DIMP analysis. <br />3 Preparation <br />Preparation for the lake sampling includes the following information, equipment and <br />materials. <br />• Information on weather conditions <br />1 U.S. Department of Defense. 1980. AD-AO82 685. Available for Defense Technical Center. Write to <br />Documents, Cameron Station, Alexandria, VA 22314. <br />2 Agency for Toxic Substances and Disease Registry. 1998. Toxicological profile for diisopropyl <br />methylphosphonate. Atlanta, GA: U.S. Department of Health and Human Services, Public Health <br />Service. <br />3 Data from SRC PhysProp Database: http://esc.s),rres.com/interkow/webprop.exe CAS=145-75-6 <br />-2-