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. ~` W.E.T. Inc. <br />Water & Environmental Testing lnc. 479 West SO North. American Fork Ulah 84003 (8011 756-839 7 <br />! Acute Whole Effluent Toxicity Report 0 ~ r 2 3 1996 <br />Fathead 1Ninnow <br />DATE: October 21, 1996 <br />PERMITTEE NAME: Western Fuels-Colorado {New Horiwn Mine) NPDS NO: OUTPACE NO: 007 <br />TESL' (Aaima!lAge): Fathead Minnow 6 days. SAMPLE (Date/Type): 10/10/96 grab. <br />DATE/1'IME TEST BEGAN: 10/11/96 7:30 p.m. DATE(C1144E TEST' COMPLETED: 1Of15146 8:30 p.m. <br />TEST CONDITIONS <br />Fathead Minnow larvae were exposed to diluted effluent as specified by permit. Survival a1 the eqd of the <br />lest period was measured and statistically evaluated for Acute Toxicity. <br />• <br />An1ui31:.Age at:Test Start: ::. 6 days. ;:: '.< <br />;. .: <br />Number of Otganisms/Dilution Volume/Repiica[es 5 organisms/200 m1/4 replicates. <br />Fogd...:.::::.:' .:.:.:...:: ::: ::.:'.:'.: :::::.::..: Brine:Shrimp. fed previous:io'tesl:and:ai48hqu.rs:::::: <br />Aeration None required. <br /> <br />Dissolged Qzygen ...::..:::.:::::-:'.:.:.::::::::::.... ;.. <br />::.. <br />,. .... <br />:Meastiiedatly:old/new:...i. ;.;:.;';:::: i:.'::::'.r:..:::.::;;: <br /> <br />:. . <br />; <br />. <br />Water Replacement . <br />Renewed every 24 hours. <br />- <br />Tem iature• ..::::.: :. ...::::. ::;i:'.:' ` ::.:.:::::::::.:.::...:.. <br />2 +. de " : .C. , {...;...:::::...:::. <br />Photo Period 16 hours ambient light/8 hours dazk. <br />H;:.:;'.:.::::.:;,, .' ...' .:,;,:;. '... ' :. .:::...::.:..:' , -..: <br />p ....... ....... :............. .... ,Measurcd:.nitiallyiynd.ati.. ...........:...:...:..:.::...:.,....... <br />,. , .... .. , 24 ho <br />DBution Water Reconstituted lab water (±15% hardness of the <br /> Receiving Water). <br />;:Receivirtg::Water;::;:::` :::::::::;: ::::: ':.::-: <br />;..:.:.. .CalamdyDcaw.(lilstoricslAata)::-' :::::.'' ::::.:::°:: <br /> <br />,::.: <br />Sample Concentrations Control, 6.75%, 12.5%, 25%, 50%, 100%a. <br />SUMMARY <br />Results: ~:;~X~;; Pass ~~ Fai] <br />There was NO significant effect oq survival. <br />Enclosed arc data sheets and statistical reports. <br />Sincerely, <br />~~ <br />Lee Rawlings <br />Water & Environmental Testing, Inc. <br />Enclosure <br /> <br />