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~~W..L~. T. Inc. <br />Water & Environmental Testirte Irtc. 479 West SO North. American Fork Ulah 84003 (801)756-8397 <br /> <br />Acute Whole Effluent Toxicity Report <br />Fathead Minnow <br />DATE: Mazch 13, 1996 <br />PERMITTEE NAME: Western Fuels-Colorado tTlcw Horiwn Mine) NPDS NO: <br />TEST (Anima!/Age): Fathead Minnow 13 days. <br />OUTFACE NO: 007 <br />SAMPLE (Dale/Type): 3/07/96. <br />DATE/I'IIVfE TESL BEGAN: 3/08/96 7:30 p.m. DATE/fIME TEST COMPLETED: 3/12/96 7:15 p.m. <br />TEST CONDTTIONS <br />Fathead Minnow larvae were exposed to diluted cfflucnt as specified by permit. Survival at the end of the <br />test period was measured and statisticallycvalualed for Acute Toxicity. <br />AnimalAge:aE Test Start .. <br />Numher of Organisms/Dilution Volume/Replicates <br />,. <br />Food:.:. :: . <br />Aeration.. , <br />Dissolved Qirygeu <br />• <br />n~re <br />13.days. 1::; :. ,. ; <br />5 organisms/200 ml/4 replicates. <br />~ ..... <br />Brine Shrimp fed.previousto test rind at 48. hours. '. <br />None required. <br />Measure:daily:oldlnew. ~ .:' <br />..:::... <br />Renewed every 24 hoursI. <br />29:± 1 degrees C. '.I ':: ':.'::: ' :. : ;:.. ;.!.; ::;::: ; <br />16 hours ambient light/8 hours dazk. <br /><Measured: initially and a( 24 hours; ::.:::::.:::.::.;.;.:.; '-:;::::" <br />Reconstituted lab water (±15%v hardness of the <br />Receiving Water). <br />Calamitya)caw (Flistorical!Rata) . '::.: '. ,!..:' .:': .';: ",:': <br />Control, 6.25%, 12.5%, 25%, 50%a, 100%. <br />SUMMARY <br />Results: X'.: Pass Fail <br />There was NO significant effect on survival. <br />Enclosed are data sheets and statistical reports. <br />~Sin~cere/l~y///~J/n/J~/~~ <br />' " /' 1 "" u 2-~/ <br />/Lee Rawlings <br />Water & Environmental Testing, Inc. <br />Enclosure <br />• <br />